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Anti cd8a pe cy7

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The Anti-CD8a-PE/Cy7 is a fluorescently-labeled antibody that binds to the CD8a surface antigen. It is commonly used in flow cytometry applications for the identification and quantification of CD8-positive cells.

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Lab products found in correlation

Anti cd45.1 fitc, by Thermo Fisher Scientific (2 mentions) Streptavidin apc efluor 780, by Thermo Fisher Scientific (2 mentions) Anti cd4 pe cy7, by Thermo Fisher Scientific (2 mentions) Anti cd150 pe, by BioLegend (2 mentions) Anti cd45.2 pe 104, by BioLegend (2 mentions) Anti cd48 alexa fluor 647 hm48 1 103416, by BioLegend (2 mentions) Anti cd45 apc efluor 780, by Thermo Fisher Scientific (2 mentions) Anti ter119 apc efluor 780, by Thermo Fisher Scientific (2 mentions) Anti b220 apc efluor 780, by Thermo Fisher Scientific (2 mentions) Cd51 pe, by Thermo Fisher Scientific (2 mentions) Anti pdgfrα cd140a apc, by Thermo Fisher Scientific (2 mentions) Anti ly6a e sca 1 fitc, by Thermo Fisher Scientific (2 mentions) Anti cd45 percp cyanine5, by Thermo Fisher Scientific (2 mentions) Anti cd11b mac 1 pe m1 70, by Thermo Fisher Scientific (2 mentions) Anti cd31 pe cy7, by Thermo Fisher Scientific (2 mentions) Anti cd45 percp cy5, by Thermo Fisher Scientific (1 mentions) Anti cd11b fitc, by Thermo Fisher Scientific (1 mentions) Anti f4 80 fitc, by BioLegend (1 mentions) Anti cd206 apc, by BioLegend (1 mentions) Anti gr 1 pe, by BD (1 mentions)

Spelling variants of this product

Anti-CD8a (18 citations) CD8a-PE (12 citations) Anti-CD8a-PE (8 citations)

3 protocols using anti cd8a pe cy7

1

Antibody Panel for Mouse Cell Analysis

Cited 5 times
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The following antibodies were used for flow cytometry: Anti-CD150-PE (TC15–12F12.2; 115904), anti-CD117-PE/Cy7 (2B8; 105814), anti-CD45.2-PE (104; 109807), anti-CD48-Alexa Fluor 647 (HM48–1; 103416) were purchased from BioLegend (San Diego, CA). anti-CD45-APC-eFluor 780 (30-F11; 47–0451-82), anti-Ter119-APC-eFluor 780 (TER-119; 47–5921-82), Streptavidin APC-eFluor 780 (47–4317-82), anti-CD31-PE/Cy7 (MEC13.3; 25–0311-82), anti-CD45.1-FITC (A20; 11–0453-85), anti-B220-APC-eFluor 780 (RA3–6B2; 47–0452-82), anti-CD4-PE/Cy7 (GK1.5; 25–0041-82), anti-CD8a-PE/Cy7 (53–6.7; 25–0081-85), CD51-PE (RMV-7; 13–0512-85), anti-PDGFRα (CD140a)-APC (APA5; 17–1401-81), anti-Ly6A/E(Sca-1)-FITC (D7; 11–5981-82), Anti-CD45-PerCp-Cyanine5.5 (30-F11; 45–0451-82), anti-CD11b (Mac-1)-PE (M1/70; 12–0112-83) were purchased from eBioscience (Thermo Fisher). Anti-lineage panel cocktail (TER-119, RB6–8C5, RA3–6B2, M1/70, 145–2C11 at 1:50 dilution) was from BD Biosciences (559971). Unless otherwise specified, all antibodies were used at a 1:100 dilution. All antibodies were anti-mouse and their specificity was validated in previous studies performed in our laboratory3 (link),8 (link),12 (link),27 (link)–29 (link).
Nakahara F., Borger D.K., Wei Q., Pinho S., Maryanovich M., Zahalka A.H., Suzuki M., Cruz C.D., Wang Z., Xu C., Boulais P.E., Ma'ayan A., Greally J.M, & Frenette P.S. (2019). Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells. Nature cell biology, 21(5), 560-567.
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2

Antibody Panel for Mouse Cell Analysis

Cited 5 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for flow cytometry: Anti-CD150-PE (TC15–12F12.2; 115904), anti-CD117-PE/Cy7 (2B8; 105814), anti-CD45.2-PE (104; 109807), anti-CD48-Alexa Fluor 647 (HM48–1; 103416) were purchased from BioLegend (San Diego, CA). anti-CD45-APC-eFluor 780 (30-F11; 47–0451-82), anti-Ter119-APC-eFluor 780 (TER-119; 47–5921-82), Streptavidin APC-eFluor 780 (47–4317-82), anti-CD31-PE/Cy7 (MEC13.3; 25–0311-82), anti-CD45.1-FITC (A20; 11–0453-85), anti-B220-APC-eFluor 780 (RA3–6B2; 47–0452-82), anti-CD4-PE/Cy7 (GK1.5; 25–0041-82), anti-CD8a-PE/Cy7 (53–6.7; 25–0081-85), CD51-PE (RMV-7; 13–0512-85), anti-PDGFRα (CD140a)-APC (APA5; 17–1401-81), anti-Ly6A/E(Sca-1)-FITC (D7; 11–5981-82), Anti-CD45-PerCp-Cyanine5.5 (30-F11; 45–0451-82), anti-CD11b (Mac-1)-PE (M1/70; 12–0112-83) were purchased from eBioscience (Thermo Fisher). Anti-lineage panel cocktail (TER-119, RB6–8C5, RA3–6B2, M1/70, 145–2C11 at 1:50 dilution) was from BD Biosciences (559971). Unless otherwise specified, all antibodies were used at a 1:100 dilution. All antibodies were anti-mouse and their specificity was validated in previous studies performed in our laboratory3 (link),8 (link),12 (link),27 (link)–29 (link).
Nakahara F., Borger D.K., Wei Q., Pinho S., Maryanovich M., Zahalka A.H., Suzuki M., Cruz C.D., Wang Z., Xu C., Boulais P.E., Ma'ayan A., Greally J.M, & Frenette P.S. (2019). Engineering a haematopoietic stem cell niche by revitalizing mesenchymal stromal cells. Nature cell biology, 21(5), 560-567.
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3

Analyzing Anti-Tumor Immune Response to P-Lipo Treatment

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To test the anti-tumor immune response of P-Lipo treatment, 104 4T1-LUC cells were injected into BALB/c female mice and treated with P-Lipo as described above. Blood was sampled from the submaxillary veins at different time points after treatment, treated with RBC lysis buffer, blocked with 5% FBS followed by staining with fluorescent antibodies, and then subjected to a CytoFlex S flow cytometer (Beckman Coulter) for immune cell detection. For T cell detection, blood samples were stained with anti-CD45-PerCP-Cy5.5 (eBioscience, 45-0451-82, 1:500), anti-CD4-FITC (BD, 553650, 1:500), anti-CD8a-PE-Cy7 (eBioscience, 14-0081-82, 1:250) antibodies. For myeloid-derived suppressor cell (MDSC) detection, blood samples were stained with anti-CD45-PerCP-Cy5.5 (eBioscience, 45-0451-82, 1:500), anti-CD11b-FITC (eBioscience, 17-01120-81, 1:500) and anti- Gr1-PE (BD, 553128, 1:500) antibodies. For macrophage detection, blood samples were stained with anti-CD45-PerCP-Cy5.5 (eBioscience, 45-0451-82, 1:500), anti-F4-80-FITC (Biolegend, 123108, 1:500), anti-CD86-Super bright 600 (Invitrogen, 63-0862-80, 1:500), and anti-CD206-APC (Biolegend, 141708, 1:500) antibodies.
Zhang L., Zhu Y., Wei X., Chen X., Li Y., Zhu Y., Xia J., Huang Y., Huang Y., Wang J, & Pang Z. (2022). Nanoplateletsomes restrain metastatic tumor formation through decoy and active targeting in a preclinical mouse model. Acta Pharmaceutica Sinica. B, 12(8), 3427-3447.
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