Quantikine human il 1β il 1f2 immunoassay

Manufactured by R&D Systems

Sourced in United States

The Quantikine Human IL-1β/IL-1f2 Immunoassay is a quantitative sandwich enzyme immunoassay designed to measure human interleukin-1 beta (IL-1β) levels in cell culture supernates, serum, and plasma.

Automatically generated - may contain errors

Lab products found in correlation

Amicon centrifugal filtration unit, by Merck Group (2 mentions) 10k membrane, by Merck Group (1 mentions) Human il 18 elisa kit, by MBL Life Science (1 mentions)

5 protocols using quantikine human il 1β il 1f2 immunoassay

Quantification of IL-1β Secretion

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Media supernatants were concentrated in Amicon centrifugal filtration units with a molecular weight limit of 10 kDa (Millipore, Billerica MA) as described previously [12 (link)]. The levels of IL-1β secreted in response to doxorubicin or asbestos with and without XMD8-92 exposure were then measured using the Human Quantikine IL-1β/IL-1F2 Immunoassay (R&D Systems, Minneapolis, MN) ELISA kit, following the manufacturer’s directions. Values are expressed as picograms of IL-1β per milliliter of supernatant initially collected.

Thompson J.K., Shukla A., Leggett A.L., Munson P.B., Miller J.M., MacPherson M.B., Beuschel S.L., Pass H.I, & Shukla A. (2017). Extracellular signal regulated kinase 5 and inflammasome in progression of mesothelioma. Oncotarget, 9(1), 293-305.

+ Open protocol

+ Expand

Quantifying Cytokine Secretion in Asbestos Exposed Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Media supernatants from in vitro experiments were concentrated in Amicon centrifugal filtration units with a molecular weight limit of 10 kDa (Millipore, Billerica MA) as described previously (Hillegass et al. 2013 (link)). The levels of IL-1β and IL-18 secreted in response to asbestos exposure were then measured using the Human Quantikine IL-1β/IL-1f2 Immunoassay (R&D Systems, Minneapolis, MN) and Human IL-18 ELISA kits (MBL, Woburn, MA) respectively following the manufacturer’s directions. Values are expressed as pg (IL-1β or IL-18)/mL of total culture supernatant initially collected.

MacPherson M., Westbom C., Kogan H, & Shukla A. (2016). Actin polymerization plays a significant role in asbestos-induced inflammasome activation in mesothelial cells in vitro. Histochemistry and cell biology, 147(5), 595-604.

+ Open protocol

+ Expand

Quantifying Human IL-1β in Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Frozen culture SN of cells treated with LPS and 16aa or 5ae were thawed and immediately tested for the presence of human IL-1β. The test was carried out by ELISA quantitative sandwich enzyme immunoassay technique (ELISA kit Quantikine, Human Il-1β/Il-1F2 immunoassay, R&D Systems, Minneapolis, USA) specific for natural and recombinant human IL-1β.

Ulgheri F., Spanu P., Deligia F., Loriga G., Fuggetta M.P., de Haan I., Chandgudge A., Groves M, & Domling A. (2021). Design, synthesis and biological evaluation of 1,5-disubstituted α-amino tetrazole derivatives as non-covalent inflammasome-caspase-1 complex inhibitors with potential application against immune and inflammatory disorders. European Journal of Medicinal Chemistry, 229, 114002.

+ Open protocol

+ Expand

Quantitative IL-1β Immunoassay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Quantikine Human IL-1β/IL-1f2 Immunoassay (R&D Systems, Minneapolis, MN, measures predominantly mature IL-1β) was used on concentrated cell medium. Medium (500 µL) was concentrated using Amcion® ultracentrifugal filters with a 10 K membrane (Millipore, Billerica, MA) by spinning at 14,000× g for 30 min. Columns were then reversed into new collection tubes and spun for 2 min at 1,000× g. IL-1β assay was performed on the concentrated medium according to the manufacturer’s instructions. A total of 500 µL of cell supernatant was concentrated. Two hundred µL samples with assay diluents were loaded into 96 well plates pre-coated with IL-1β antibody. Values are expressed as pg/mL of IL-1β from the original supernatant (non-concentrated).

Dragon J., Thompson J., MacPherson M, & Shukla A. (2015). Differential susceptibility of human pleural and peritoneal mesothelial cells to asbestos exposure. Journal of cellular biochemistry, 116(8), 1540-1552.

+ Open protocol

+ Expand

Quantifying IL-1β and IL-18 in Cell Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Quantikine Human IL-1β/IL-1f2 Immunoassay (R&D Systems, Minneapolis, MN, measures predominantly mature IL-1β) was used on concentrated cell medium, prepared as described earlier, and the assay performed according to the manufacturer’s instructions as previously published [3 (link)]. IL-18 release was measured using the Human IL-18 ELISA kit (MBL International, Woburn, MA, measures predominantly active IL-18, 0.7% pro IL-18). Values were expressed as pg/mL of IL-1β or IL-18 from the original supernatant (non-concentrated).

Westbom C., Thompson J.K., Leggett A., MacPherson M., Beuschel S., Pass H., Vacek P, & Shukla A. (2015). Inflammasome Modulation by Chemotherapeutics in Malignant Mesothelioma. PLoS ONE, 10(12), e0145404.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!