Nahco3

Manufactured by Beijing Chemical Works

Sourced in China

NaHCO3 is a chemical compound with the formula NaHCO3. It is a white crystalline or powdery substance commonly known as sodium bicarbonate or baking soda. NaHCO3 is a versatile compound with various industrial and household applications.

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5 protocols using nahco3

Analytical Standards for Bioactive Compounds

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We purchased standards for quercetin (purity=98.0%) and vanillic acid (purity=98.0%) from Shanghai Shidande Standard Technical Service Co., Ltd. (Shanghai, China). HPLC-grade methanol (purity≥99.9%) was purchased from Fisher Chemical (Waltham, USA). All water used in this research was double distilled. Other reagents and chemicals were all analytical grade. MgCl₂·6H₂O (purity≥98.0%), KCl (purity≥99.5%), NaCl (purity≥99.5%), CaCl₂ (purity≥96.0%), D-Glucose ([α]20D: +52.5° - +53.0°), NaHCO₃ (purity≥99.5%), NaH₂PO₄ (purity≥99.0%), concentrated hydrochloric acid (ω%36.0–38.0%), and acetic acid (purity≥99.5%) were supplied by Beijing Chemical Works (Beijing, China). Ethyl carbamate (trade name: urethane) was purchased from Tianjin Guangfu Fine Chemical Research Institute (Tianjin, China). Finally, 0.9% sodium chloride injection solution was obtained from Shijiazhuang Pharmaceutical Siyao Co., Ltd. (Shijiazhuang, China).

Yao Y., Mi W., Cao G., Yang R., Chen H., Liu Y., Zou H, & Yan Y. (2020). The Absorption Characteristics of Nonvolatile Components in a Water Extraction From Amomi fructus as Determined by In Situ Single-Pass Intestinal Perfusion and High-Performance Liquid Chromatography. Frontiers in Pharmacology, 11, 711.

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Mycotoxin Detection Using Aptamers

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Mycotoxin standards including AFB₁, AFM₁, OTA, ZEN, FB₁, and DON were purchased from Pribolab (Singapore, Singapore). A total of seven oligonucleotide sequences against AFB₁ and each corresponding complementary ssDNA were synthesized and purified through HPLC by Sangon Biotechnology Co., Ltd. (Shanghai, China), and these aptamers were listed in Table 1 and their corresponding complementary sequences (Seq) was listed in Table S1. The fluorescent dye PicoGreen was purchased from Invitrogen (Carlsbad, CA, USA). The areca nuts, lotus seed, malt, Radix Paeoniae Alba, and Chinese yam were collected from China, India, Philippines, and Indonesia. Acetonitrile, methanol, Tris-HCl, EDTA-2Na, Na₂CO₃, NaHCO₃, and NaCl was analytical grade and purchased from Beijing Chemical Works (Beijing, China). PestiCarb (GCB 120-400 Mesh), octadecylsilane (C18) and Cleanert primary secondary amine (PSA, 40–60 μm) were from Beijing Agela Technologies Inc. Deionized water was purified using a Milli-Q system (Millipore, CA, USA).

Zhang C., Dou X., Zhang L., Sun M., Zhao M., OuYang Z., Kong D., Antonio F.L, & Yang M. (2018). A Rapid Label-Free Fluorescent Aptasensor PicoGreen-Based Strategy for Aflatoxin B1 Detection in Traditional Chinese Medicines. Toxins, 10(3), 101.

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Hemoglobin-Based Antioxidant Assay

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All the chemicals used were of analysis grade without further purification. Hemoglobin (Hb, from bovine blood) was purchased from Sigma-Aldrich. Thiamine, L-ascorbic acid (Vc) and folic acid (VB₉) were obtained from Guoyao Chemical Research Institute (Shenyang, China). NaCl, KCl, NaHCO₃, NaHPO₄, NaH₂PO₄, NaHSO₄, NaOH, glucose and hydrogen peroxide (H₂O₂, 30%) were purchased from Beijing Chemical Works (Beijing, China). 1,2-diaminobenzene (OPD) and CuSO₄ were purchased from Tianjin Guangfu Fine Chemical Research Institute (Tianjin, China). The water used in the experiments was purified. The ρ of the water was 18 MΩ·cm.

Zou H., Zhang Y., Zhang C., Sheng R., Zhang X, & Qi Y. (2020). Fluorometric Detection of Thiamine Based on Hemoglobin–Cu3(PO4)2 Nanoflowers (NFs) with Peroxidase Mimetic Activity. Sensors (Basel, Switzerland), 20(21), 6359.

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Antioxidant Evaluation of Rutin and Gallic Acid

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Rutin, gallic acid, α‐amylase, bile salts (a mixture of sodium cholate and sodium deoxycholate), pepsin from pig gastric mucosa, pancreatin from pig pancreas, and 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical were purchased from Sigma‐Aldrich. HPLC grade acetonitrile, trifluoroacetic acid (TFA, 99%), methanol, NaHCO_3, and other analytical reagents were purchased from Beijing Chemical Works.

Zhang Q., Xing B., Sun M., Zhou B., Ren G, & Qin P. (2020). Changes in bio‐accessibility, polyphenol profile and antioxidants of quinoa and djulis sprouts during in vitro simulated gastrointestinal digestion. Food Science & Nutrition, 8(8), 4232-4241.

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Synthesis of Metal-Organic Frameworks

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Terephthalic acid (H₂BDC, 99% purity), methyl sulfoxide-d6 (99.8% atom% D purity), cinnamyl alcohol (98% purity), 3-nitrobenzyl alcohol (>99% purity), 4-methylbenzyl alcohol (98% purity), and TEMPO (98% purity) were supplied by Beijing InnoChem Science & Technology Co., Ltd. CO₂ ( >99.95% purity), water, N₂ ( >99.95% purity), Ar (>99.999% purity), and O₂ (99.99% purity) were purchased from Beijing Analysis Instrument Factory. TEA (A. R. grade), ethanol (A. R. grade), methanol (A. R. grade), DMF (A. R. grade), acetonitrile (A. R. grade), Na₂CO₃ (A. R. grade), and NaHCO₃ (A. R. grade) were supplied by Beijing Chemical Works. CoCl₂·6H₂O (A. R. grade), Cu(NO₃)₂·3H₂O (A. R. grade), and NiCl₂·6H₂O (A. R. grade) were brought from China National Medicines Co., Ltd. 1,4-Naphthalenedicarboxylic acid (H₂NDC, 99% purity), 2-naphthalenemethanol (98% purity), 1-phenylethanol (97% purity), 1-(4-methoxyphenyl)ethanol (95% purity), and furfuryl alcohol (98% purity) were purchased from J&K Scientific Co., Ltd. Benzyl alcohol was supplied by Alfa Aesar China Co., Ltd. All these materials were used directly without further purification.

Zhang F., Zhang J., Zhang B., Zheng L., Cheng X., Wan Q., Han B, & Zhang J. (2020). CO2 controls the oriented growth of metal-organic framework with highly accessible active sites. Nature Communications, 11, 1431.

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