Flow cytometry analysis of B7H6 expression on tumor cells were performed by staining cells with anti-B7H6 mAbs,8 (link) followed by DyLight 649-conjugated goat anti-mouse IgG (clone: Poly4053, Biolegend, San Diego, CA). For staining of mouse cells, the samples were pre-incubated with FcR block antibody (anti-mouse CD16/CD32, 2.4G2; Bio X Cell, Lebanon, NH), and for staining of human cells, samples were pre-incubated with human gamma globulin (Cohn fraction, Sigma-Aldrich). Samples were analyzed by Accuri C6 cytometer (BD Biosciences, San Jose, CA) and data was analyzed by Accuri or FlowJo software (TreeStar Inc, Ashland, OR).
Human gamma globulin cohn fraction
Manufactured by Merck Group
Human gamma globulin (Cohn fraction) is a laboratory product derived from human plasma. It contains a concentrated mixture of immunoglobulins, primarily immunoglobulin G (IgG). The core function of this product is to serve as a source of IgG for various research and diagnostic applications.
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2 protocols using human gamma globulin cohn fraction
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B7H6 Expression Analysis by Flow Cytometry
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B7H6 Expression Analysis by Flow Cytometry
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Flow cytometry analysis of B7H6 expression on tumor cells were performed by staining cells with anti-B7H6 mAbs,8 (link) followed by DyLight 649-conjugated goat anti-mouse IgG (clone: Poly4053, Biolegend, San Diego, CA). For staining of mouse cells, the samples were pre-incubated with FcR block antibody (anti-mouse CD16/CD32, 2.4G2; Bio X Cell, Lebanon, NH), and for staining of human cells, samples were pre-incubated with human gamma globulin (Cohn fraction, Sigma-Aldrich). Samples were analyzed by Accuri C6 cytometer (BD Biosciences, San Jose, CA) and data was analyzed by Accuri or FlowJo software (TreeStar Inc, Ashland, OR).
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