Mouse rat pyy elisa kit

The blood glucose concentration was measured using One Touch Ultra Test Strips (One Touch^® Ultra^®, Life Scan, Milpitas, CA, USA). The concentrations of plasma total cholesterol (Lab Assay™ Cholesterol, Wako, Tokyo, Japan), NEFAs (Lab Assay™ NEFA, Wako, Tokyo, Japan), plasma and hepatic TG (Lab Assay™ Triglyceride, Wako, Tokyo, Japan), plasma PYY (Mouse/Rat PYY ELISA Kit, Wako, Tokyo, Japan), GLP-1 (GLP-1 Active ELISA Kit, Merck Millipore, Darmstadt, Germany), and insulin (Insulin ELISA KIT (RTU), Shibayagi, Gunma, Japan) were measured following the manufacturer’s instructions. For plasma GLP-1 measurement, the plasma sample was treated with a dipeptidyl peptidase IV inhibitor (Merck Millipore, Darmstadt, Germany) to prevent the degradation of active GLP-1. SCFA levels in the plasma and caecum were determined following a modification of a previously described protocol^{49 (link)}. Herein, the SCFA-containing ether layers were collected and pooled for gas chromatography-mass spectrometry analysis using a GCMS-QP2010 Ultra system (Shimadzu, Kyoto, Japan). The calibration curves for SCFAs were constructed, and the concentration of each SCFA in the samples was evaluated over a specified concentration range.

Blood glucose concentrations were measured using a One Touch Ultra (LifeScan, Milpitas, CA, USA). The concentrations of plasma and fecal triglycerides (LabAssay Triglyceride; Wako Pure Chemical Co. Ltd., Osaka, Japan), total cholesterol (LabAssay Cholesterol; Wako Pure Chemical Co. Ltd.), PYY (Mouse/Rat PYY ELISA Kit, Wako), GLP-1 (active) ELISA kit (Merck Millipore, Billerica, MA, USA), and an insulin ELISA Kit (Shibayagi, Gunma, Japan) were measured according to manufacturer instructions. For GLP-1 measurement, plasma samples and culture media were treated with a dipeptidyl peptidase IV (DPP-IV) inhibitor (Merck Millipore) to prevent degradation of active GLP-1.
For GTT, 24-h-fasted obese mice were given 1.5 mg of glucose/gram of body weight intraperitoneally (i.p.). For ITT, 3-h-fasted obese mice were administered human insulin (0.75 mU/g by i.p.; Sigma-Aldrich, St. Louis, MO, USA). Plasma glucose concentration was monitored before injection and at 15-, 30-, 60-, and 120-min post-injection.

Blood glucose was assessed using a portable glucometer with compatible glucose test strips (OneTouch ® Ultra ®, LifeScan, Milpitas, CA). Plasma cholesterol (LabAssay™ Cholesterol, Wako, Tokyo, Japan), NEFA (LabAssay™ NEFA, Wako), insulin [Insulin ELISA KIT (RTU), Shibayagi], PYY (Mouse/Rat PYY ELISA Kit, Wako) and triglyceride (LabAssay™ Triglyceride, Wako) levels were measured using commercial assay kits following manufacturer’s instructions. Plasma levels of GLP-1 were determined by enzyme-linked immunosorbent assay (ELISA) (GLP-1 (Active) ELISA KIT, Shibayagi, Gunma, Japan) following treatment with dipeptidyl peptidase IV (DPP-IV) inhibitor (Merck Millipore, Darmstadt, Germany), which prevents the degradation of active GLP-1.