Cdna synthesis kit
The cDNA Synthesis Kit is a laboratory product designed to convert RNA into complementary DNA (cDNA) molecules. The kit provides the necessary reagents and protocols to perform this reverse transcription process, which is a fundamental step in various molecular biology and genomic analysis techniques.
Lab products found in correlation
24 protocols using cdna synthesis kit
Evaluating FTO and IRX3 Gene Expression in Blood
Evaluating FTO and IRX3 Expression
Quantifying MDM2 Expression in HCT-116 Cells
Total RNA was extracted from HCT-116 cells (untreated and treated with apatinib and piperine at different concentrations as mentioned above) using an RNA extraction kit based on the kit protocol (GeneAll, South Korea). The First-strand cDNAs were synthesized by GeneAll cDNA synthesis kit (GeneAll, South Korea) and utilized as templates to perform real-time PCR, following the manufacturer’s instruction. The MDM2 gene expression was measured by real-time PCR using a Real Q Plus 2x Master Mix Green (Ampliqon, Denmark). Real time-PCR via cDNAs and specific primers were carried out in annealing temperature at 57°C for 30 seconds. Relative MDM2 expression was normalized to β-actin housekeeping gene and calculated by 2-ΔΔCt. Error bars in control and treated groups show the Standard deviation.
Quantitative Analysis of Gene Expression
Quantitative Analysis of Stemness Genes
Cardiac mRNA Expression Analysis
Adipogenic and Osteogenic Gene Expression
Primer sequences for adipogenic and osteogenic differentiations.
Gene name | Sequences | Ta |
---|---|---|
PPAR-γ2 | F- CTATTGACCCAGAAAGCGAT R- CGTAATGTGGAGTAGAAATGC | 54 |
C/EBP-α | F- GGTGCGTCTAAGATGAGGGG R- CATTGGAGCGGTGAGTTTGC | 55 |
RUNX2 | F- CTCCCCAGGCCAAACACA R- TCCGAGGGCTACCACCTTGA | 55 |
Osteocalcin | F- CACCGAGACACCATGAGAGC R- CTGCTTGGACACAAAGGCTGC | 54 |
RPII | F- GCACCATCAAGAGAGTCCAGT R- ATTTGATGCCACCCTCCGTCA | 57 |
RNA Extraction and qRT-PCR Analysis
Quantitative Analysis of Blood RNA
Quantitative Gene Expression Analysis
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