Beta tubulin ab6046

Manufactured by Abcam

Sourced in United States

Beta Tubulin (ab6046) is a recombinant protein that can be used as a standard or control in quantitative assays. It is a component of the cytoskeleton and plays a role in cell division and intracellular transport.

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Lab products found in correlation

Flag f3165, by Merck Group (1 mentions) Hrp conjugated goat anti rabbit igg sa00001 2, by Proteintech (1 mentions) Hrp conjugated goat anti mouse igg sa00001 1, by Proteintech (1 mentions) Fastprep 24 homogenizer, by MP Biomedicals (1 mentions) Gapdh, by Merck Group (1 mentions) Sc 397, by Santa Cruz Biotechnology (1 mentions) Itgb4, by Santa Cruz Biotechnology (1 mentions) Pai 1 sc 5297, by Santa Cruz Biotechnology (1 mentions) Medical x ray film, by Konica Minolta (1 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions) Westernbright ecl reagent, by Advansta (1 mentions) Iblot dry blotting system, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Ab6046 (231 citations)

6 protocols using beta tubulin ab6046

Antibody Generation and Validation Protocol

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Primary antibodies against the following proteins or peptides were used: GBP2 (11854-1-AP, Proteintech, Wuhan, China); Flag (F3165, Sigma-Aldrich, Burlington, MA, USA); beta Tubulin (ab6046, Abcam, Waltham, MA, USA). Secondary antibodies used were as follows: HRP-conjugated goat anti-mouse IgG (SA00001-1, Proteintech, China); HRP-conjugated goat anti-rabbit IgG (SA00001-2, Proteintech, China). Polyclonal EVM H3L antiserum were generated in our laboratory. In brief, the EVM H3L target sequences were used to construct the pET30a-H3L recombinant prokaryotic expression plasmids. The plasmids were then transformed into Rosetta competent cells and induced with IPTG. Finally, antiserum was prepared by immunizing rabbits with the purified proteins.

Gao Z., Meng Z., He X., Chen G., Fang Y., Tian H., Zhang H, & Jing Z. (2023). Guanylate-Binding Protein 2 Exerts GTPase-Dependent Anti-Ectromelia Virus Effect. Microorganisms, 11(9), 2258.

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Multiparametric Analysis of Redox Regulators

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All the chemicals were purchased from Sigma-Aldrich, Germany unless mentioned otherwise. Antibodies for flow cytometry: BV421 Mouse Anti-Human CD11b/MAC-1, clone ICRF44; PerCP-Cy™5.5 Mouse Anti-Human CD14, clone M5E2; PE Mouse Anti-Human CD16, Clone B73.1; FITC Mouse Anti-Human CD62L, clone SK11 and BV421 Mouse IgG1, k Isotype Control, clone X40 were bought from BD Biosciences, Sweden. APC Mouse anti-human CD68, clone Y1/82A and AmCyan Mouse anti-human HLA-DR, clone L243 were bought from Biolegend, UK. Antibodies for western blot and their dilutions were as follows: RARα (Sc-551, C-20), 1:500, Santa Cruz Biotechnology; PML (A301-168A-1), 1:500, Bethyl Laboratories; PU.1 (GTX 45005), 1:1000, GeneTex; Grx1, 1:200, own production; Grx2 (GTX 112094), 1:1000, GeneTex; Grx3, 1:1000, own production; Grx5, (GTX 45005), 1:250, GeneTex; TrxR1 (GTX 110589), 1:1000, GeneTex; TrxR2 (ab58445), 1:750, Abcam; Trx1, 1:3000, own production; Trx2 1:3000, own production; Beta-actin (A4700), 1:1000, Sigma; Beta-tubulin (Ab6046), 1:3000, Abcam; FOXO3A (GTX 62705), 1:1000, GeneTex.

Misra S., Selvam A.K., Wallenberg M., Ambati A., Matolcsy A., Magalhaes I., Lauter G, & Björnstedt M. (2016). Selenite promotes all-trans retinoic acid-induced maturation of acute promyelocytic leukemia cells. Oncotarget, 7(46), 74686-74700.

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Aortic Protein Expression Analysis

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Protein lysates were prepared in RIPA buffer with protease inhibitor from sections of the abdominal aorta extending from the renal arteries to the iliac bifurcation using a FastPrep-24 homogenizer (MP Biomedicals, LLC, Santa Ana, CA). Protein was quantitated using a Pierce BCA protein assay kit and 40 μg aliquots were electrophoresed on 4–15% polyacrylamide reducing gels using standard methods (Thermo Fisher, Waltham, MA). Protein was then electroblotted to nitrocellulose membranes and stained with Ponceau red to assess quality. Membranes were blocked and incubated with primary antibodies. MYHII (ab53219) and beta-tubulin (ab6046) were obtained from Abcam (Abcam, Cambridge, MA). Tet2 (21207-AP) was obtained from Proteintech (Proteintech, Rosemont, IL). Appropriate HRP-conjugated secondary antibodies were obtained (Abcam, Cambridge, MA). Proteins were visualized using the upperSignal WestPico Chemiluminescent Substrate kit (Thermo Fisher, Waltham, MA). The relative quantity of proteins of interest was determined after normalization with beta-tubulin using ImageJ Version 1.5 (U. S. National Institutes of Health, Bethesda, Maryland, USA).

Gelinne A., Brown L., Ko N.L., Osol G, & Brown S. (2018). EPIGENETIC BASIS FOR ABDOMINAL AORTIC VASCULAR SMOOTH MUSCLE ADAPTATION TO PREGNANCY. Journal of vascular research, 55(5), 319-327.

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Immunoblotting for EMT Markers

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Antibodies against Cav1 (D46G3; 1/2000), E-cadherin (24E10; 1/2000), Slug (C19G7; 1/1000), pSmad3 (S423/425, 9520; 1/250), and CDK6 (3136; 1/500) were from CST. Pai-1(sc-5297; 1/500), Smad2/3, (sc-8332; 1/500), p21 (sc-397; 1/500), and ITGB4 (sc-9090, 1/500) were from Santa-Cruz. Beta-tubulin (ab6046; 1/10 000), ITGB1 (EP1041Y; 1/5000), and PSF (ab38148; 1/1000) were from Abcam. Actin was from Sigma-Aldrich (A1978; 1/10 000), GAPDH from Millipore (MAB374; 1/3000), and DSP from Bethyl (A303-355A; 1/1000).

Pellinen T., Blom S., Sánchez S., Välimäki K., Mpindi J.P., Azegrouz H., Strippoli R., Nieto R., Vitón M., Palacios I., Turkki R., Wang Y., Sánchez-Alvarez M., Nordling S., Bützow A., Mirtti T., Rannikko A., Montoya M.C., Kallioniemi O, & del Pozo M.A. (2018). ITGB1-dependent upregulation of Caveolin-1 switches TGFβ signalling from tumour-suppressive to oncogenic in prostate cancer. Scientific Reports, 8, 2338.

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Tip60 Protein Expression Analysis

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Whole cell extracts were prepared from indicated cells lines by re-suspending cell pellets and incubating in Lysis Buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 0.1% Tween-20, 1 mM EDTA, 10% glycerol, 1 mM DTT, 1 mM PMSF, 1 mM NaVO4, 1 mM aprotinin and 1 mM pepstatin) at 4 °C for 1 h. 30 μg of total protein was separated using SDS-PAGE gels and transferred to nitrocellulose membranes. Chemiluminescence was detected using SuperSignal West Pico Chemiluminescent Substrate® (Thermo Scientific) and medical X-ray film (Konica Minolta). Antibodies: Tip60 (K-17: sc-5727) Santa Cruz and beta-Tubulin (ab6046) Abcam.

McGuire A., Casey M.C., Shalaby A., Kalinina O., Curran C., Webber M., Callagy G., Holian E., Bourke E., Kerin M.J, & Brown J.A. (2019). Quantifying Tip60 (Kat5) stratifies breast cancer. Scientific Reports, 9, 3819.

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Immunoblotting of Type I Collagen

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Whole-cell lysates were prepared by lysing cells with RIPA buffer (50 mM Tris–HCl (pH 8.0), 150 mM NaCl, 5 mM EDTA, 1% NP40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate). Equal amounts of protein were loaded on 4% to 20% Tris–Glycine gels from Thermo Fisher Scientific for electrophoresis. Gels were then electrotransferred onto nitrocellulose membranes using the iBlot Dry Blotting system (Thermo Fisher Scientific) and subjected to immunoblotting using primary antibodies specific for human type I type I collagen (#1310-01) from Southern Biotech (Birmingham, AL, USA); beta-tubulin (#ab6046) and m-Cherry (#ab125096) from Abcam (Cambridge, MA). Membranes were then incubated with appropriate secondary antibodies from Abcam and subjected to enhanced chemiluminescence detection using WesternBright ECL Reagent (Advansta, USA).

Wong H.H., Seet S.H., Bascom C.C., Isfort R.J, & Bard F. (2020). Red-COLA1: a human fibroblast reporter cell line for type I collagen transcription. Scientific Reports, 10, 19723.

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