Tumor tissue was collected and immediately fixed in 10% neutral-buffered formalin at 4 °C overnight before dehydration and paraffin embedding. Antibodies used for immunohistochemistry were anti-ER (M7047, 1:300, Agilent) and anti-Ki-67 (M7240, 1:400, Agilent). Primary antibodies were detected using biotinylated IgG secondary antibodies (Agilent, 1:400), using streptavidin-HRP (Agilent) for amplification of signal followed by the addition of 3,3′-diaminobenzidine (Sigma) substrate. Images were scanned using Leica Aperio Slide Scanner (Leica Biosystems) and analyzed using QuPath software to differentiate tumor tissue from stroma and necrosis, and to quantify Ki-67 positivity in tumor tissue.
Biotinylated igg secondary antibodies
Manufactured by Agilent Technologies
Biotinylated IgG secondary antibodies are laboratory reagents used in immunological techniques. They are designed to bind to primary antibodies, facilitating the detection and visualization of target analytes in samples. The biotinylation enables further signal amplification through interactions with streptavidin or avidin-conjugated detection systems.
Automatically generated - may contain errors
Lab products found in correlation
M7047, by Agilent Technologies (4 mentions)
M7240, by Agilent Technologies (4 mentions)
Leica aperio slide scanner, by Leica Biosystems (3 mentions)
Streptavidin hrp, by Agilent Technologies (3 mentions)
3 3 diaminobenzidine (dab), by Merck Group (2 mentions)
Nanozoomer, by Hamamatsu Photonics (1 mentions)
4 protocols using biotinylated igg secondary antibodies
1
Immunohistochemical Analysis of Tumor Samples
2
Immunohistochemical Profiling of Tumor Samples
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Tumour tissue was harvested and immediately fixed in 10% neutral buffered formalin at 4 °C overnight before dehydration and paraffin embedding. Antibodies used for IHC were anti-ER (M7047, 1:300, Agilent) and anti-Ki67 (M7240, 1:400, Agilent). Primary antibodies were detected using biotinylated IgG secondary antibodies (Agilent, 1:400), using streptavidin-HRP (Agilent) for amplification of signal followed by the addition of 3,3′-diaminobenzidine (Sigma) substrate. Images were scanned using Leica Aperio Slide Scanner (Leica Biosystems) and analysed using QuPath software to differentiate tumour tissue from stroma and necrosis, and to quantify Ki-67 positivity in tumour tissue.
3
Immunohistochemical Quantification of Tumor Markers
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Antibodies used for IHC include anti-AR (#SC-27190, 1:1000, Santa Cruz), anti-ER (M7047, 1:300, Agilent), anti-Ki67 (M7240, 1:400, Agilent) and anti-SEC14L2 (#271902, 1:1000, Santa Cruz). These primary antibodies were detected with biotinylated IgG secondary antibodies (Agilent, 1:400), using streptatvidin-HRP (Agilent) for amplification of signal followed by the addition of 3,3'-diaminobenzidine (Sigma) substrate for visualization of signal. Images were scanned using NanoZoomer (Hamamatsu). Proliferation indices were determined by the proportion of Ki-67 positive cells for each tumor by manual counting of >1000 cells from three random 80× magnification fields (Dowsett et al. 2011) (link). Quantification of staining intensity was performed using ImageJ from at least three representative images at 80×.
4
Immunohistochemical Analysis of Tumor Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tumour tissue was harvested and immediately fixed in 10% neutral buffered formalin at 4°C overnight before dehydration and paraffin embedding. Antibodies used for IHC were anti-ER (M7047, 1:300, Agilent) and anti-Ki67 (M7240, 1:400, Agilent).
Primary antibodies were detected using biotinylated IgG secondary antibodies (Agilent, 1:400), using streptavidin-HRP (Agilent) for amplification of signal followed by the addition of 3,3′-diaminobenzidine (Sigma) substrate. Images were scanned using Leica Aperio Slide Scanner (Leica Biosystems) and analysed using QuPath software to differentiate tumour tissue from stroma and necrosis, and to quantify Ki-67 positivity in tumour tissue.
Primary antibodies were detected using biotinylated IgG secondary antibodies (Agilent, 1:400), using streptavidin-HRP (Agilent) for amplification of signal followed by the addition of 3,3′-diaminobenzidine (Sigma) substrate. Images were scanned using Leica Aperio Slide Scanner (Leica Biosystems) and analysed using QuPath software to differentiate tumour tissue from stroma and necrosis, and to quantify Ki-67 positivity in tumour tissue.
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