Spss 17.0 statistics

The hDPSC (3 × 10 4 cells per cm 2 ) were initially cultured in 12-well culture dishes (3 × 10 4 cells per cm 2 ) and stored in an incubator at 37°C and 5% CO 2 for 1 day. Thereafter, the cells were cultured in low-glucose DMEM (LG-DMEM) containing 10% FBS and 0.2% primocin and placed in direct contact with sterilized discs of bulk fill composite resins. he stem cells were also cultured with no composite discs to create positive controls. The number of viable cell were measured using a cell proliferation assay kit on days 1, 7, 14, and 21 of co-culture. Briefly, WST-1 dye (Cell Proliferation Reagent WST-1, Roche) was added to the culture medium of each well (1:10, 10 μL WST-1 dye to 100 μL medium) at the indicated time points. After storage in an incubator at 37°C and 5% CO 2 for 150 min, the absorbance was measured using a monochromatic microplate reader system (VersaMax Microplate Reader, Molecular Devices, Sunnyvale, CA, USA) at 450 nm wavelength.
The data was analyzed using IBM SPSS 17.0 statistics program for Windows. The mean, median, minimum value, maximum value, and standard deviations were calculated. Significance was tested with the Mann-Whitney U Test.