Temed

The following antibodies were used: monoclonal mouse anti-human fibronectin antibody number 42042, purchased from QED Bioscience Inc., San Diego, California, USA, and goat anti-rabbit immunoglobulin G number A5420, conjugated with horseradish peroxidase, obtained from Sigma-Aldrich, Germany. The following reagents were applied: sodium metaperiodate and hydrazide LC-biotin obtained from Thermo Scientific, USA; standard fibronectin from human plasma, DMSO (dimethyl sulfoxide), sodium dodecyl sulfate, Triton X-100, Brilliant blue R250, glycine, Immobilon P membranes, dithiothreitol, Tween 20 (polyoxyethylene sorbitan monolaurate), and TMB (3.3′,5,5′-tetramethylbenzidine), all supplied by Sigma-Aldrich, Germany; Sephadex G-25 obtained from Pharmacia, Sweden; HEPES (4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid) supplied by Fluka, Germany; BLOT-QuickBlocker purchased from Millipore, USA; TEMED purchased from ICN Biomedicals, USA; streptavidin-coated sensor chip (SAP) obtained from XanTec Bioanalytics, Germany. All of the remaining, applied reagents were supplied by POCh Gliwice, Poland.

The following reagents were used: 100 µg/ml HEL (Sigma), 100 µg/ml BSA (Euromedex), 40% polyacrylamide (PAA, Biorad), 2% bis-poylacrylamide (Biorad), 3-aminopropyltrimethoxysilane (Sigma), 0.2 µm Alexa647 Fluospheres (Thermo Fisher, F8807), Sigmacote (Sigma), ammonium persulfate (Sigma), TEMED (ICN Biomedicals), Sulfo-SANPAH (Thermo Fisher), and the Alexa555 protein labeling kit (A30007, Molecular Probes). The following primary antibodies were used: rabbit anti-HEL (Abcam,1/100), human anti-GFP (Institut Curie, 1/200), rabbit Anti-phospho-Cortactin (pTyr⁴⁶⁶) (SAB4504373, Sigma-Aldrich, 1:200), rabbit anti Clathrin (Cell Signalling 4796, 1:50), Alexa Fluor 488 Phalloidin (A12379 Invitrogen 1:200), Alexa Fluor 647-conjugated anti-phalloidin (Thermo Fisher, 1/200), anti-myosin IIA heavy chain (Covance, 1/500). The following secondary antibodies were used: Alexa Fluor 488-conjugated goat anti-rabbit IgG (Life Technologies, 1/200), Alexa Fluor 488-conjugated goat anti-human IgG (Life Technologies,1/200), Alexa Fluor 405 Goat anti-Rabbit IgG (H + L) (A31556 Thermofisher 1:200), Alexa Fluor 546 Goat anti-Mouse IgG1 (A21123 Thermofisher 1:200).