For the migration assay, cells were grown in 10% FCS medium. Normoxic control experiments were performed at 21% O2, and hyperoxic experiments were performed at 80% O2 conditions. Dimethyl sulfoxide (DMSO) was used as the solvent control as the steroids were dissolved in DMSO. Cells were treated with E2 (17β-estradiol; Sigma-Aldrich, Taufkirchen, Germany), DHEA (5-andostene-3b-ol-17-one; Sigma-Aldrich), DHEA+E2, 16α-OH-DHEA (5-androsten-3β, 16α-diol-17-one; Steraloids, Newport, RI), 16α-OH-DHEA+E2, adiol (5-androsten-3β, 17β-diol; Steraloids), and adiol+E2. All steroids were added to a final concentration of 100 nM.
16α oh dhea
Manufactured by Steraloids
16α-OH-DHEA is a chemical compound that can be used as a reference standard or intermediate in analytical and research applications. It is a derivative of dehydroepiandrosterone (DHEA), a naturally occurring steroid hormone. The core function of 16α-OH-DHEA is to serve as a tool for the identification, quantification, and study of related compounds in various scientific and analytical contexts.
Automatically generated - may contain errors
Lab products found in correlation
Adiol, by Steraloids (1 mentions)
17β estradiol e2, by Merck Group (1 mentions)
7 oxo dhea, by Steraloids (1 mentions)
D3 dhea, by Steraloids (1 mentions)
D4 cortisol, by CDN Isotopes (1 mentions)
7α oh dhea, by Steraloids (1 mentions)
2 hydrazinopyridine, by Merck Group (1 mentions)
Ammonium formate, by Merck Group (1 mentions)
Trifluoroacetic acid, by Merck Group (1 mentions)
Methyl tert butyl ether (mtbe), by Merck Group (1 mentions)
Diethyl ether, by Merck Group (1 mentions)
Lc ms grade methanol, by Merck Group (1 mentions)
2 protocols using 16α oh dhea
1
Steroid Effects on Cell Migration
2
Steroid Extraction and Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cortisol, cortisone and dehydroepiandrosterone (DHEA) were purchased form Koch-Light Laboratories LTD (Colnbrook, Great Britain), 7α-OH-DHEA, 7β-OH-DHEA, 7-oxo-DHEA, 16α-OH-DHEA and D3-DHEA were from Steraloids (Newport, USA). D4-Cortisol was from CDN isotopes (Ponte-Claire, Canada).
2-hydrazinopyridine, ammonium formate, methyl tertbutyl ether and trifluoroacetic acid were from Sigma-Aldrich (St. Louis, USA). LC-MS grade methanol, water and diethyl ether were from Merck AG (Darmstadt, Germany). The physiological solution (0.9 % sodium chloride) was from B-Braun (Melsungen AG, Germany). [1,2,6,7-3H]Cortisol, specific radioactivity 3.04 TBq/mmol was from Amesrham Biosciences, Inc.
(Amersham, UK).
2-hydrazinopyridine, ammonium formate, methyl tertbutyl ether and trifluoroacetic acid were from Sigma-Aldrich (St. Louis, USA). LC-MS grade methanol, water and diethyl ether were from Merck AG (Darmstadt, Germany). The physiological solution (0.9 % sodium chloride) was from B-Braun (Melsungen AG, Germany). [1,2,6,7-3H]Cortisol, specific radioactivity 3.04 TBq/mmol was from Amesrham Biosciences, Inc.
(Amersham, UK).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!