For analysis of surface markers, cells were stained in PBS containing 2% BSA. The following fluorescent‐labeled antibodies (purchased from BD Biosciences) were used: CD13 PE (652820), CD33 APC (652807), MPO FITC (652821), and CD45 PerCP (652803). Flow cytometry data were collected using Canto‐I (BD Biosciences) cytometers and analyzed with FlowJo V10 software.
Mpo fitc
Manufactured by BD
The MPO FITC is a lab equipment product used for fluorescent labeling and detection. It utilizes FITC (Fluorescein Isothiocyanate) as the fluorescent dye. The core function of the MPO FITC is to enable fluorescent-based analysis and imaging applications in a laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mpo fitc
1
Immunophenotyping of Hematological Cells
2
Multi-Color Flow Cytometry Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For image stream analysis, cell suspensions were incubated in two antibody cocktails.
The extra-cellular antibody cocktail contained Ly6G-PE (BioLegend, San Diego, CA; 1:100), CD11b-efluor 780 (Life Technologies, Carlsbad, CA, US; 1:100), CD45-Pacific blue (Biolegend, San Diego, CA, US; 1:200), and Live/Dead aqua (ThermoFisher Scientific, Waltham, MA; 1:1000) for 30 minutes at 4ºC. Cells were then fixed with 0.1% paraformaldehyde solution and permeabilized with 0.5% tween solution in 1x phosphate buffer saline (PBS). Cells were then incubated with intracellular antibody cocktail containing MPO-FITC (BD Biosciences, Franklin Lakes, NJ; 1:100). Following a final rinse, cells were resuspended in a 1xPBS solution containing the DNA dye, DRAQ5 (ThermoFisher Scientific, Waltham, MA; 1:1000). Cell suspensions were then analyzed using an Amnis ImageStream X Mark II cytometer (Cytek Biosciences, Bethesda, MD) and analyzed with the Image Data Exploration and Analysis Software (IDEAS).
The extra-cellular antibody cocktail contained Ly6G-PE (BioLegend, San Diego, CA; 1:100), CD11b-efluor 780 (Life Technologies, Carlsbad, CA, US; 1:100), CD45-Pacific blue (Biolegend, San Diego, CA, US; 1:200), and Live/Dead aqua (ThermoFisher Scientific, Waltham, MA; 1:1000) for 30 minutes at 4ºC. Cells were then fixed with 0.1% paraformaldehyde solution and permeabilized with 0.5% tween solution in 1x phosphate buffer saline (PBS). Cells were then incubated with intracellular antibody cocktail containing MPO-FITC (BD Biosciences, Franklin Lakes, NJ; 1:100). Following a final rinse, cells were resuspended in a 1xPBS solution containing the DNA dye, DRAQ5 (ThermoFisher Scientific, Waltham, MA; 1:1000). Cell suspensions were then analyzed using an Amnis ImageStream X Mark II cytometer (Cytek Biosciences, Bethesda, MD) and analyzed with the Image Data Exploration and Analysis Software (IDEAS).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!