Gsk621

Manufactured by Merck Group

Sourced in Canada

GSK621 is a laboratory instrument designed for conducting various scientific experiments and analyses. It is a versatile piece of equipment that can be used in a wide range of research and development applications. The core function of GSK621 is to provide precise and reliable data collection and analysis capabilities to support scientific investigations.

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Lab products found in correlation

3 protocols using gsk621

Compound Screening for Drug Discovery

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Chemicals used in this study were from the following sources: nilotinib (Selleckchem, S1033), imatinib (Selleckchem, S2475), torin1 (Selleckchem, S2827), Gefitinib (MedChemExpress, HY-50895), GSK621 (Sigma, SML2003), EX229 (MedChemExpress, HY-112769), BC1618 (MedChemExpress, HY-134656), bafilomycin A1 (Selleckchem, S1413), MK2206 (Selleckchem, S1078), SRT1720 (Apexbio, A4180–10), FR180204 (Selleckchem, S7524), PLX-4720 (Selleckchem, S1152). PEG300 (Selleckchem, S6704), DMSO (Sigma, D2650).

Zheng W., Chang I.C., Limberis J., Budzik J.M., Zha B.S., Howard Z., Chen L, & Ernst J.D. (2023). Mycobacterium tuberculosis resides in lysosome-poor monocyte-derived lung cells during chronic infection. bioRxiv.

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Compound Screening for Drug Discovery

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Zheng W., Chang I.C., Limberis J., Budzik J., Zha B.S., Howard Z., Chen L, & Ernst J. (2023). Mycobacterium tuberculosis resides in lysosome-poor monocyte-derived lung cells during chronic infection. Research Square.

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Modulation of AMPK Signaling in ZIKV Infection

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The antibodies used in the study were: anti-phospho (Thr172)-AMPKα, anti-phospho acetyl-CoA carboxylase (Ser79) (ACC), anti-AMPKα, anti-HK 2 (Cell Signaling Technology, Beverly, MA), anti-flavivirus 4G2 IgG antibody (EMD Millipore) anti-ZIKV NS3 (Genetax), anti-GLUT1 (Santa Cruz Biotechnologies), at dilutions of 1:1000, and anti-β-actin (Sigma Aldrich) at a dilution of 1:5000. The secondary antibodies used were goat anti-rabbit/mice-IgG-HRP (Bio-Rad, Hercules, CA), rabbit anti-mouse Alexa 594 (Invitrogen). The drugs used in the study were AICAR (1 mM) (Toronto Research Chemicals, Inc. (ON, Canada), Compound C (10 μM) (Toronto Research Chemicals, Inc. (ON, Canada), GSK621 (10 μM) (Sigma-Aldrich), 2-DG (1 mM) (Sigma-Aldrich) and Metformin (20 mM) (Ranbaxy Laboratories (Princeton, NJ). For AMPK inhibition and activation studies, cells were pre-treated with respective drugs for an hour prior to virus challenge, removed during adsorption and then added again in fresh media and kept for the entire duration of the experiment.

Singh S., Singh P.K., Suhail H., Arumugaswami V., Pellett P.E., Giri S, & Kumar A. (2020). Adenosine monophosphate-activated protein kinase (AMPK) restricts Zika virus replication in endothelial cells by potentiating innate antiviral responses and inhibiting glycolysis. Journal of immunology (Baltimore, Md. : 1950), 204(7), 1810-1824.

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