Protein samples were extracted with RIPA buffer. The extracted total protein was separated by SDS-PAGE and transferred to PVDF membranes. Following incubation in blocking buffer for 1 h at room temperature, the membrane was incubated at 4°C overnight with Ptch1 antibody (Abcam, ab109096), Foxo3 antibody (Thermo Fisher Scientific, FA1-14171), SQSTM1/p62 antibody (Abcam, ab56416), and LC3B (D11) XP antibody (Cell Signaling Technology, 3868S). The results were visualized using Beyo ECL Plus substrate and exposed to X-ray film. β-actin was used for normalization.
Lc3b d11 xp antibody
Manufactured by Cell Signaling Technology
The LC3B (D11) XP® Rabbit mAb is a recombinant monoclonal antibody that recognizes the microtubule-associated protein 1 light chain 3 beta (LC3B) protein. LC3B is a widely used marker for autophagosomes and is involved in the autophagy process.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lc3b d11 xp antibody
1
Protein Expression Analysis by Western Blot
2
Western Blot Analysis of Protein Markers
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Protein samples were extracted with RIPA buffer. The extracted total protein was separated by SDS-PAGE and transferred to PVDF membranes. Following incubation in blocking buffer for 1 h at room temperature, the membrane was incubated at 4°C overnight with Ptch1 antibody (Abcam, ab109096), Foxo3 antibody (Thermo, FA1-14171), SQSTM1 / p62 antibody (Abcam, ab56416), and LC3B (D11) XP antibody (Cell Signaling Technology, 3868S). The results were visualized using Beyo ECL Plus substrate and exposed to X-ray lm. β-actin was used for normalization.
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