17β estradiol

Manufactured by Thermo Fisher Scientific

Sourced in United Kingdom, United States

17β-estradiol is a steroid hormone that is a type of estrogen. It is used as a reference standard in analytical laboratories for the identification and quantification of 17β-estradiol in various samples.

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Lab products found in correlation

17β estradiol, by Merck Group (3 mentions) β actin, by Cell Signaling Technology (2 mentions) Ethanol, by Thermo Fisher Scientific (1 mentions) Puradisc, by Cytiva (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Rabbit anti erβ, by Santa Cruz Biotechnology (1 mentions) Mouse anti erα, by Santa Cruz Biotechnology (1 mentions) Rabbit anti erα, by Santa Cruz Biotechnology (1 mentions) Hrp conjugated secondary antibody, by Cell Signaling Technology (1 mentions) Irdye conjugated secondary antibody, by LI COR (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Rabbit anti pten, by Cell Signaling Technology (1 mentions) 2 7 dichlorodihydrofluorescein diacetate dcfh da, by Merck Group (1 mentions) Complete dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions) P foxo3a, by Cell Signaling Technology (1 mentions) Dihydroethidium dhe, by Merck Group (1 mentions) P akt, by Cell Signaling Technology (1 mentions) Insulin, by Thermo Fisher Scientific (1 mentions) Igf 1, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Estradiol (9 citations)

8 protocols using 17β estradiol

Comparative Evaluation of Anabolic Compounds

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Five different compounds were compared in this study: lactoferrin, lithium chloride, 17β-estradiol (oestrogen), icariin, and menaquinone-4 (MK-4). Each was applied at three concentrations spanning three orders of magnitude in order to encompass the range reported as anabolic in the literature (Table 1). The vehicle concentration was controlled for all conditions.
To create stock solutions, lactoferrin from bovine colostrum (cat# L4765, ~90 kDa) was dissolved in BM at 5 mg/mL, and lithium chloride (cat# L9650) was dissolved in BM at 1 M. Oestrogen, icariin, and MK-4 have poor solubility in aqueous solutions such as culture media. Therefore, 17β-estradiol (cat# E2758) was dissolved in ethanol (Fisher Scientific, UK), and then diluted in BM to a final concentration of 10 μM 17β-estradiol and 2% ethanol. Icariin and MK-4 were both dissolved in dimethyl sulfoxide (DMSO) at 10 mM. All stock solutions were sterilised by 0.2 μM filtration using ultra-low adsorption polyether sulfone filters (Whatman® Puradisc), divided into single-use aliquots and stored at −20 °C.

Owen R., Bahmaee H., Claeyssens F, & Reilly G.C. (2020). Comparison of the Anabolic Effects of Reported Osteogenic Compounds on Human Mesenchymal Progenitor-Derived Osteoblasts. Bioengineering, 7(1), 12.

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Inducible Silencing in C. roseus Hairy Roots

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To express the silencing hairpin (Zct1hp or GFPhp), transgenic C. roseus hairy roots were induced with 5μM 17β-estradiol (Fisher Scientific) on day 26 (late-exponential growth phase). Previously, induction with 5μM 17β-estradiol showed strong and tightly regulated expression of GFP in control transgenic C. roseus hairy roots [31 ]. Stock solutions (5mM) of 17β-estradiol were prepared in dimethyl sulfoxide (DMSO, Sigma Aldrich), and 50μL of stock solution was added to 50mL of culture media to achieve a final concentration of 5μM 17β-estradiol. Uninduced cultured were treated with 50μL of DMSO.
Cultures were elicited with MJ (≥ 95%, Sigma Aldrich) 24 h after induction with 17β-estradiol. Stock solutions were prepared in ethanol (200 proof, ACS/USP grade, Pharmco-AAPER) and added to 50mL of culture media to achieve final concentrations of 250μM or 1000μM MJ. Cultures were harvested 8, 24, and 48 h after MJ addition for mRNA analysis or after 3, 5, and 7 days for TIA metabolite analysis. C. roseus hairy roots were blotted and flash-frozen in LN₂.

Rizvi N.F., Weaver J.D., Cram E.J, & Lee-Parsons C.W. (2016). Silencing the Transcriptional Repressor, ZCT1, Illustrates the Tight Regulation of Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus Hairy Roots. PLoS ONE, 11(7), e0159712.

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Estrogen Receptor Signaling Pathway Analysis

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Rabbit anti-PTEN, phospho-PTEN (S380, T382, T383), AKT, β-actin and GAPDH as well as mouse anti-phospho-AKT (S473) were purchased from Cell Signaling Technologies (Danvers, MA). Mouse anti-protein kinase CK2α was purchased from Millipore (Billerica, MA). Mouse anti-ERα, rabbit anti-ERα and rabbit anti-ERβ were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX). Rabbit anti-GPER antibody was supplied by GenScript USA Inc. (Piscataway, NJ). IRDye conjugated secondary antibodies used in western immunoblotting are from LI-COR Biosciences (Lincoln, NE) while HRP conjugated secondary antibodies used in western immunoblotting are from Cell Signaling Technologies (Danvers, MA). 17β-estradiol was diluted in 200 proof ethanol (Fisher Scientific, Pittsburgh, PA) and used at a final concentration of 10 nM (Sigma-Aldrich, St. Louis, MO).

Scully M.M., Palacios-Helgeson L.K., Wah L.S, & Jackson T.A. (2014). Rapid Estrogen Signaling Negatively Regulates PTEN Activity Through Phosphorylation in Endometrial Cancer Cells. Hormones & Cancer, 5(4), 218-231.

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Estradiol Signaling Pathway Analysis

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17β-estradiol, dihydroethidium (DHE), and 2′,7′-dichloro dihydro fluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich (St. Louis, MO, United States). Antibodies (rabbit anti-human) against ERα, Akt, p-Akt, Foxo3a, p-Foxo3a, and β-actin were purchased from Cell Signaling Technology (Beverly, MA, United States). The 17β-estradiol was dissolved in dimethyl sulfoxide (DMSO) to prepare a stock solution at a concentration of 50 mg/ml, and was stored at −20°C. Complete Dulbecco’s modified Eagle medium (DMEM; Gibco, Gaithersburg, MD, United States) was added to dilute the 17β-estradiol to the appropriate concentrations prior to use.

Guo Y., Cai X., Lu H., Li Q., Zheng Y., Lin Z., Cheng Z., Yang M., Zhang L., Xiang L, & Yang X. (2021). 17β-Estradiol Promotes Apoptosis of HepG2 Cells Caused by Oxidative Stress by Increasing Foxo3a Phosphorylation. Frontiers in Pharmacology, 12, 607379.

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MCF7 Breast Cancer Cell Culture Protocol

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The MCF7 breast carcinoma cell line was obtained from the ATCC and cultured in DMEM (GIBCO) containing 10 % serum [7.5 % calf serum (CS) + 2.5 % fetal bovine serum (FBS)] (GIBCO), in a humidified 37 °C incubator with 5 % CO₂. Insulin was purchased from Life Technologies and used at a concentration of 10 ug/ml. IGF-1 was purchased from PeproTech and used at a concentration of 10 ng/ml. 17β-estradiol was purchased from Sigma-Aldrich and used at a concentration of 10⁻⁸ M. For experiments using 17β-estradiol, cells were cultured in phenol red-free DMEM (GIBCO) supplemented with 10 % charcoal-stripped FBS (Hyclone). Cells were treated for 4 h with Insulin, IGF-1 or 17β-estradiol prior to fixation. Construction of the human mier1α sequence (GenBank: AY124188) in the CS3 + MT vector has been described previously [1 (link)]. Transient transfection, confocal microscopy, antibodies used and Z-stack analysis were performed as described in [6 , 7 ]. Subcellular localization was scored as ‘nuclear’ if the nucleus was intensely stained, with little or no cytoplasmic staining; ‘cytoplasmic’ if staining was primarily in the cytoplasm, with little or no staining in the nucleus; ‘whole cell’ if both the nucleus and cytoplasm were stained [6 ]. Statistical analysis was performed using a two-sided Fisher’s exact test.

Li S., Paterno G.D, & Gillespie L.L. (2015). Insulin and IGF-1, but not 17β-estradiol, alter the subcellular localization of MIER1α in MCF7 breast carcinoma cells. BMC Research Notes, 8, 356.

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Porcine Oocyte Maturation and Embryo Culture

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All reagents were purchased from Nacalai Tesque (Kyoto, Japan), unless otherwise stated. For culture of OGCs, we used α‐MEM (Sigma‐Aldrich, SaintLouis, Missouri, USA) supplemented with 10 mmol/L taurine, 0.1 mAU/mL follicle‐stimulating hormone (Kawasaki Mitaka, Tokyo, Japan), 2% (w/v) polyvinylpyrrolidone‐360 (Sigma‐Aldrich), 2 mmol/L hypoxanthine (Sigma‐Aldrich), 1% (w/v) insulin transferrin selenium (Gibco BRL, Paisley, UK), 1 µg/mL 17β‐estradiol, 3 mg/mL BSA, and antibiotics. The in vitro maturation (IVM) medium was Medium 199 (Gibco) supplemented with 10% (v/v) porcine follicular fluid (pFF), 0.5 mmol/L L‐cysteine, 0.9 mmol/L sodium pyruvate, 1 mmol/L l‐glutamine, 10 ng/mL epidermal growth factor, 5% (v/v) fetal calf serum, 10 IU/mL equine chorionic gonadotropin (ASKA Pharma Co. Ltd, Tokyo, Japan), and 10 IU/mL human chorionic gonadotropin (Fuji Pharma Co. Ltd, Tokyo, Japan). In vitro culture (IVC) of embryos and oocyte activation was conducted in porcine zygote medium 3 (PZM3).12 In vitro culture of OGCs and oocyte maturation was performed at 38.5°C in an atmospheric condition of 5% CO₂ and 95% air, whereas in vitro embryo culture was performed at 38.5°C in an atmospheric condition of 5% O₂, 5% CO₂, and 90% N₂.

Shibahara H., Ishiguro A., Shirasuna K., Kuwayama T, & Iwata H. (2019). Follicular factors determining the developmental competence of porcine oocyte. Reproductive Medicine and Biology, 18(3), 256-262.

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Estrogen Modulation of Gut Barrier Function

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Gelfoam (Gelfoam absorbable gelatin sponge, Pfizer Inc., NY, USA) were distributed into 12-well tissue culture plates. Gut samples from postmenopausal females including 3 whites and 3 Africa Americans (3 mm × 3 mm) were placed on the Gelfoam and then cultured at 37°C with RPMI-1640 medium (Gibco) containing penicillin (100 unit/ml), streptomycin (100 µg/ml) and 10% charcoal stripped FBS (Gibco). The tissues were treated with different concentrations (0, 0.2, 2 and 20 ng/ml) of 17β-estradiol (Alfa Aesar, Tewksbury, USA) for 24 h. Samples were extracted total RNA by EZNA Total RNA Kit (Omega Bio-tek, Doraville, GA, USA). cDNA was synthesized from 5 µg of each RNA sample using the SuperScript III First-Strand Synthesis System (Invitrogen) followed by PCR. Primer sequences, reaction conditions, and optimal cycle numbers of PCR were described previously [35 (link)]. The expression level of ZO-1 was analyzed using comparative threshold cycle method (2^−ΔΔCT) with GAPDH as an internal reference [36 (link)]. The expression level of medium without estrogen treatment was set as 1. The experiment was performed three times.

Zhou Z., Zhang L., Ding M., Luo Z., Yuan S., Bansal M.B., Gilkeson G., Lang R, & Jiang W. (2017). Estrogen decreases tight junction protein ZO-1 expression in human primary gut tissues. Clinical immunology (Orlando, Fla.), 183, 174-180.

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Synthesis and Characterization of Organic Compounds

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Aniline (ANI, Merck, Darmstadt, Germany) was distilled under reduced pressure, and metanilic acid or m-aminobenzenesulfonic acid (MSAN, Acros Organics, Geel, Belgium) was purified by recrystallization twice from deionized (DI) water. Testosterone (≥98.0%), progesterone (≥99.0%), urea (minimum ≥98.0%), creatinine (minimum ≥99.0%), and ethanol were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). 17β-Estradiol (≥98.0%) was from Alfa-Aesar (Ward Hill, MA, USA). Ammonium peroxydisulfate (APS) used as the initiator was from Wako (Osaka, Japan). The ITO-coated glass substrates (~10 Ω·cm⁻²) were from Merck. Deionized water (18.2 MΩ), produced by a PURELAB Ultra (ELGA, High Wycombe, UK), was used in the preparation of buffers and for rinse solutions. All chemicals were used as received unless otherwise mentioned.

Liu K.H., O’Hare D., Thomas J.L., Guo H.Z., Yang C.H, & Lee M.H. (2020). Self-assembly Synthesis of Molecularly Imprinted Polymers for the Ultrasensitive Electrochemical Determination of Testosterone. Biosensors, 10(3), 16.

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