The Annexin V-FITC Apoptosis Cardiomyocyte apoptosis was quantified by annexin V/PI apoptosis assay (BD, CA, USA). Harvested cells were re-suspended in binding buffer after being washed with cold PBS. In 100 µl of cell suspension, annexin V and PI were introduced. After a 15-minute incubation period at room temperature in the dark, 400 µl of binding buffer was introduced to each tube. The assessment was done with a fluorescence-activated cell sorting equipment with FlowJo software.
Annexin 5 pi apoptosis assay
Manufactured by BD
Sourced in United States
The Annexin V/PI apoptosis assay is a laboratory technique used to detect and quantify the process of programmed cell death, known as apoptosis. It utilizes two fluorescent dyes, Annexin V and Propidium Iodide (PI), to distinguish between cells undergoing early apoptosis and late apoptosis or necrosis. The assay provides a reliable method for the analysis of cell viability and cell death mechanisms.
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2 protocols using annexin 5 pi apoptosis assay
1
Annexin V-FITC Apoptosis Assay for Cardiomyocytes
2
Quantification of Cardiomyocyte Apoptosis
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Cardiomyocyte apoptosis was quantified by annexin V/PI apoptosis assay (BD, CA, USA). Briefly, harvested cells were rinsed with cold PBS and resuspended in binding buffer. Annexin V and PI were added to 100 μl of cell suspension. After incubating at room temperature for 15 min protected from light, 400 μl binding buffer was added to each tube. The analysis was conducted using a fluorescence-activated cell sorting machine with FlowJo software.
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