Axiovision release 4

Manufactured by Zeiss

Sourced in Germany, Canada

AxioVision Release 4.8 is a software suite for image acquisition, processing, and analysis. It provides a user-friendly interface for controlling Zeiss microscopes and cameras, as well as tools for tasks such as image segmentation, object measurement, and 3D visualization.

Automatically generated - may contain errors

Lab products found in correlation

Axiocam hrc, by Zeiss (7 mentions) Axiophot microscope, by Zeiss (7 mentions) Axiocam, by Zeiss (6 mentions) Axio observer z1, by Zeiss (6 mentions) Axiocam mrm camera, by Zeiss (5 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (5 mentions) Axioskop 2 plus, by Zeiss (4 mentions) Axio imager, by Zeiss (4 mentions) Axiocam hrc digital camera, by Zeiss (4 mentions) Axiocam mrm, by Zeiss (4 mentions) Lsm 510 meta, by Zeiss (4 mentions) Lsm 700 confocal microscope, by Zeiss (4 mentions) Axio observer a1, by Zeiss (3 mentions) Sk 4100, by Vector Laboratories (3 mentions) Axioskop fluorescence microscope, by Zeiss (3 mentions) Axiocam hrm, by Zeiss (3 mentions) Axioplan 2 imaging, by Zeiss (3 mentions) Apotome, by Zeiss (3 mentions) Axiophot hb0 50, by Zeiss (3 mentions) Goat anti mouse igg texas red, by Thermo Fisher Scientific (3 mentions)

96 protocols using axiovision release 4

Multicolor Chromosome Imaging Protocol

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For the two-dimensional image acquisition, an Axioplan 2 microscope (Carl Zeiss Ltd., Toronto, ON, Canada) with a 63 ×/1.4 oil objective lens (Carl Zeiss Ltd., Toronto, ON, Canada) and the ISIS-FISH imaging system 5.0 SR 3 (Metasystems Group Inc., Boston, MA) were used. The chromosomal counterstain was visualized with the help of a 4’6’-diamidino-2-phenylindole filter. To detect the four regions of chromosome 11 that were labeled with different fluorochromes (DEAC, FITC, Gold, and Texas Red), narrow band-pass filters were used (Chroma Technologies) as described by our group previously.
3D image acquisition was conducted using an AxioImager Z2 microscope (Carl Zeiss Inc. Canada) equipped with the same filters and an AxioCam MRm (Carl Zeiss Inc. Canada), combined with the Axiovision Release 4.8 software (Carl Zeiss Inc. Canada). Z-stacks of 80 slices, with 200-nm axial distance and 102-nm lateral pixel size, were acquired to reconstruct a 3D image. Using Axiovision Release 4.8 software (Carl Zeiss Inc. Canada), deconvolution was conducted with the constrained iterative algorithm (Schaefer et al., 2001).

Schmälter A.K., Righolt C.H., Kuzyk A, & Mai S. (2015). Changes in Nuclear Orientation Patterns of Chromosome 11 during Mouse Plasmacytoma Development. Translational Oncology, 8(5), 417-423.

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Collagen Fiber Analysis in Cornea

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After transmittance measurements, the corneas were fixed with 4% buffered paraformaldehyde and embedded in paraffin. Sections 5-μm thick were stained with Picrosirius-red (0.1% Sirius red in saturated aqueous picric acid for 1 h) (Junqueira et al., 1979) . Sections were examined under an Axiophot microscope (Zeiss Axiophot HBO-50; Carl Zeiss, Oberkochen, Germany) with polarized light. Photomicrographs were captured using the AxioCam HRc Digital Camera and Axiovision release 4.8 software (Carl Zeiss) under the same plane of polarization. Polarization colors are associated with different collagen fiber thicknesses, packing density, and spatial arrangement (Dayan et al., 1989) . Orange to red colors were correlated with thick collagen fibers that were tightly packed and well aligned. Yellowish-green colors were correlated with thinner fibers that were less tightly packed.
Other 5-μm sections were stained with a modification of the Gomori's Silver Impregnation technique to demonstrate the presence of collagen type III fibers [54] . The sections were examined under an Axiophot microscope (Zeiss Axiophot HBO-50; Carl Zeiss, Oberkochen, Germany) and photomicrographs were obtained with an AxioCam HRc Digital Camera and Axiovision release 4.8 software (Carl Zeiss).

Lorenzo-Martín E., Gallego-Muñoz P., Mar S., Fernández I., Cidad P, & Martínez-García M.C. (2019). Dynamic changes of the extracellular matrix during corneal wound healing. Experimental eye research, 186.

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Quantifying Lung Transplant Rejection Markers

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Formalin fixed paraffin embedded tissue from CLAD explant (n=4, all BOS) and normal donor (n=2) was sectioned 24–48hrs before use and stored at 4°C until time of processing. Probes of interest including Areg, EGFR, PPIB (positive control) and negative control were purchased from Advanced Cell Diagnostics (Newark, CA). Slides were processed according to manufacturer instructions (supplement). Probes were developed using chromogenic red kit. Images were obtained using a Zeiss Observer.Z1 inverted fluorescent microscope (Carl Zeiss, Inc., Gottingen, Germany) equipped with a digital camera and processed using AxioVision Release 4.6.3 (Carl Zeiss) Software.

Todd J.L., Kelly F.L., Nagler A., Banner K., Pavlisko E.N., Belperio J.A., Brass D., Weigt S.S, & Palmer S.M. (2019). Amphiregulin contributes to airway remodeling in chronic allograft dysfunction after lung transplantation. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 20(3), 825-833.

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Mammary Gland Morphometric Analysis

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The number of mammary ductal branch points, ductal outgrowth length and number of terminal end buds (TEBs) were determined from mammary whole mount images taken under bright field microscope using SteREOLumar, a V12 microscope with Axiocam Zeiss MRc camera and AxioVision Release 4.6.3 software (Carl Zeiss AG, Germany). Morphometric analysis was performed using Image J 1.44 software. To determine the number of branch points, grids were overlaid on images and all resolvable branch points were counted at the leading edge of advancing ducts. The number of branch points was then averaged to yield the mean number in each grid. Mammary ductal length was measured as the length from the primary duct to the leading edge of the duct. The number of TEBs was determined by counting the number of TEBs with areas ≥ 0.03 mm² at the leading edge of advancing ducts. Only TEBs with areas ≥ 0.03 mm² and having distinct bulbous feature were counted as TEB structures. This is to differentiate TEBs from terminal end ducts 23 (link).

Shrestha S., Sun Y., Lufkin T., Kraus P., Or Y., Garcia Y.A., Guy N., Ramos P., Cox M.B., Tay F, & Lin V.C. (2015). Tetratricopeptide Repeat Domain 9A Negatively Regulates Estrogen Receptor Alpha Activity. International Journal of Biological Sciences, 11(4), 434-447.

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Puromycin Sensitivity Dose Response

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The puromycin dose response curves were performed by seeding 5×10³ Bge cells per well into 6-well plates and 2 days later puromycin dihydrochloride (Life Technology, USA) was added to the culture medium at final concentrations of 0.05, 0.5 and 5 μg/ml. Given that puromycin is supplied at 10 mg/ml in 20 mM HEPES buffer (pH 7.2 – 7.5), cells cultured in 10 μM HEPES (but without puromycin) served as controls. The cells were observed every day under bright light using a Zeiss Axio Observer A.1 inverted microscope fitted with a digital camera (AxioCam ICc3, Zeiss, USA). Manipulation of digital images, which was limited to insertion of scale bars, adjustments of brightness and contrast, cropping and the like, was undertaken with the AxioVision release 4.6.3 software (Zeiss). The experiment was repeated three times.

Rinaldi G., Yan H., Nacif –Pimenta R., Matchimakul P., Bridger J., Mann V.H., Smout M.J., Brindley P.J, & Knight M. (2015). Cytometric analysis, genetic manipulation and antibiotic selection of the snail embryonic cell line Bge from Biomphalaria glabrata, the intermediate host of Schistosoma mansoni. International journal for parasitology, 45(8), 527-535.

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Insulin Immunohistochemistry in Rat Pancreas

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An immunohistochemistry of insulin was performed to identify β-cells by using guinea pig polyclonal anti-insulin antibody (A0564, Dako) diluted 1:100 in rat pancreas sections (4 µm), as previously described (13 (link)). Images of insulin-positive pancreatic β-cells in Langerhans islets were captured in all fields from each animal at a total magnification of 200X, and their area and number were assessed using the software AxioVision Release 4.6.3 (Zeiss, Göttingen, Germany).

Otero A., Becerril S., Martín M., Cienfuegos J.A., Valentí V., Moncada R., Catalán V., Gómez-Ambrosi J., Burrell M.A., Frühbeck G, & Rodríguez A. (2023). Effect of guanylin peptides on pancreas steatosis and function in experimental diet-induced obesity and after bariatric surgery. Frontiers in Endocrinology, 14, 1185456.

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Histopathological Analysis of Colon Post-Transplantation

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On day 28 after transplantation, animals were sacrificed. Hematoxylin-eosin-stained organ sections from individual mice were coded without reference to mouse type and prior treatment and detailed analysis of changes in colon was performed in a blinded fashion as described previously [34 (link)]. Analysis was performed by light microscopy (Axioskop 2 plus, Carl Zeiss GmbH, Jena, Germany). Photographs of histopathology were acquired by AxioCam HRc (Carl Zeiss GmbH) and processed with AxioVision Release 4.6.3 (Carl Zeiss GmbH, Jena, Germany).

Bouazzaoui A., Bogari N.M., Al-Allaf F.A., Ekram S.N., Athar M., Dannoun A., Schubert T., Syed S.N., Youssef A.R., Alqahtani M, & Abdellatif A.A. (2023). Anti-E. coli Immunoglobulin Yolk (IgY): Reduction of pathogen receptors and inflammation factors could be caused by decrease in E. coli load. Heliyon, 9(3), e13876.

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Measuring Adipocyte Surface Area in SCWAT

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The adipocyte surface area (CSA) of adipocytes in SCWAT was measured as previously described [64 (link)]. Briefly, sections (6 µm) of 4% formaldehyde-fixed and paraffin-embedded SCWAT biopsies were stained with H&E. Images of three fields per section from each animal were captured with the 40× objective, and the adipocyte CSA from, at least, 100 cells/section were measured using AxioVision Release 4.6.3 software (Zeiss, Göttingen, Germany).

Becerril S., Tuero C., Cienfuegos J.A., Rodríguez A., Catalán V., Ramírez B., Valentí V., Moncada R., Unamuno X., Gómez-Ambrosi J, & Frühbeck G. (2022). Improved Adipose Tissue Function after Single Anastomosis Duodeno-Ileal Bypass with Sleeve-Gastrectomy (SADI-S) in Diet-Induced Obesity. International Journal of Molecular Sciences, 23(19), 11641.

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Measuring Brown Adipocyte Cell Surface Area

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The cell surface area (CSA) of brown adipocytes was measured as previously described [23 (link)]. Briefly, biopsies of BAT were fixed in 4% formaldehyde, embedded in paraffin, cut into sections of 4 μm and stained with hematoxylin–eosin. Images of three fields per section from each animal were captured with the 20× objective, and the brown adipocyte CSA from, at least 100 cells/section was measured using the software AxioVision Release 4.6.3 (Zeiss, Göttingen, Germany).

Frühbeck G., Méndez-Giménez L., Becerril S., Ramírez B., Hernández-Pardos A.W., Cienfuegos J.A., Valentí V., Moncada R., Catalán V., Gómez-Ambrosi J., da Silva I.V., Soveral G, & Rodríguez A. (2023). Increased Aquaporin-7 Expression Is Associated with Changes in Rat Brown Adipose Tissue Whitening in Obesity: Impact of Cold Exposure and Bariatric Surgery. International Journal of Molecular Sciences, 24(4), 3412.

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Histological Evaluation of Colitis in Mice

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Full-length colons of experimental mice were removed, flushed of fecal contents, opened longitudinally, and placed in Bouin’s fixative for 24 hours. Tissues were embedded in paraffin, cut to 3 µm, and stained with H&E. Disease severity was evaluated by a trained pathologist (Wei Xin, M.D.) in a blinded fashion using an established histologic scoring system for colitis^{34 (link)}. Images were obtained on an Axiophot microscope, captured on an Axiocam and assembled using Axiovision Release 4.5 (Carl Zeiss, Inc., Thornwood, NY).

Goodman W.A., Omenetti S., Date D., Di Martino L., De Salvo C., Kim G.D., Chowdhry S., Bamias G., Cominelli F., Pizarro T.T, & Mahabeleshwar G.H. (2016). KLF6 contributes to myeloid cell plasticity in the pathogenesis of intestinal inflammation. Mucosal immunology, 9(5), 1250-1262.

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