Donkey anti mouse cy5 affinipure

Manufactured by Jackson ImmunoResearch

Donkey anti-mouse–Cy5 AffiniPure is a secondary antibody conjugated with the fluorescent dye Cy5. It is designed to detect and bind to mouse primary antibodies, allowing for fluorescent labeling and visualization of target proteins or molecules in various applications, such as immunohistochemistry, flow cytometry, and Western blotting.

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Lab products found in correlation

Donkey anti rabbit alexa fluor 647, by Jackson ImmunoResearch (2 mentions) Donkey anti rabbit cy3, by Jackson ImmunoResearch (2 mentions) Mouse anti e cadherin, by BD (2 mentions) Draq5, by Cell Signaling Technology (2 mentions) Alexa fluor 488 donkey anti rabbit, by Jackson ImmunoResearch (1 mentions) Hoechst, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Roche (1 mentions) Mouse anti p63, by Abcam (1 mentions) Mouse anti muc5ac, by Abcam (1 mentions) Mouse anti c jun, by Abcam (1 mentions)

Spelling variants of this product

Anti-mouse Cy5 (30 citations) Cy5-conjugated Affinipure donkey anti-mouse antibody (4 citations) Cy5-conjugated AffiniPure donkey anti-mouse IgG (4 citations)

2 protocols using donkey anti mouse cy5 affinipure

Immunostaining of Epithelial Markers

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The following antibodies and chemicals were used: mouse anti-p63 (Abcam, ab375), rabbit anti-p63 (Abcam, ab53039), mouse anti-E-cadherin (BD Biosciences, 610181), rabbit anti-Ki67 (Abcam, ab16667), rat anti-Ki67–FITC (eBioscience, 11-5698), rabbit anti-KRT5 (Abcam, ab52635), rabbit anti-KRT5–Alexa488 (Abcam, ab193894), mouse anti-KRT7 (Santa Cruz, sc-23876), rabbit anti-KRT7 (Abcam, ab181598), rabbit anti-KRT7–Alexa555 (Abcam, ab209601), rabbit anti-CSTA (Sigma, HPA001031), rabbit anti-AGR2 (Proteintech, 12275-1-AP), mouse anti-MUC5B (Abcam, ab77995), rabbit anti-GDA (Sigma, HPA019352), Hoechst (Sigma, B2261), rabbit anti-KRT17 (Abcam, ab109725), mouse anti-KRT19 (Abcam, ab7754), mouse anti-KRT18 (Abcam, ab668), donkey anti-rabbit–Alexa Fluor 488 (Jackson Immuno Research, 711-546-152), donkey anti-rabbit–Cy3 (Jackson Immuno Research, 711-166-152), donkey anti-rabbit–Alexa Fluor 647 (Jackson Immuno Research, 647 711-605-152), donkey anti-mouse–Cy5 AffiniPure (Jackson Immuno Research, 715-175-151), Draq5 (Cell Signaling, 4085), γ-secretase inhibitor XX (DBZ) (Calbiochem 565789) and p38 inhibitor SB202190 (Sigma, S7067). Antibody concentrations and the link to validation by the provider are shown in Supplementary Table 15.

Chumduri C., Gurumurthy R.K., Berger H., Dietrich O., Kumar N., Koster S., Brinkmann V., Hoffmann K., Drabkina M., Arampatzi P., Son D., Klemm U., Mollenkopf H.J., Herbst H., Mangler M., Vogel J., Saliba A.E, & Meyer T.F. (2021). Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia. Nature Cell Biology, 23(2), 184-197.

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Immunofluorescence Analysis of Epithelial Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies and chemicals were used: mouse anti-E-Cadherin (BD Biosciences, 610181), mouse-anti-E-Cadherin-488 (BD Biosciences, 560061), rabbit anti-cytokeratin 5-Alexa488 (Abcam, ab193894), mouse anti-p63 (Abcam, ab375), rabbit anti-cytokeratin 7 (Abcam, ab181598), rabbit anti-cytokeratin 7-Alexa555 (Abcam, ab209601), rabbit anti-cytokeratin 8 (Abcam, ab59400), mouse anti-MUC5AC (Abcam, ab212636), rabbit anti-cytokeratin 17 (Abcam, ab109725), mouse anti-c-Jun (Abcam, ab280089), mouse anti-cytokeratin 6 (Abcam, ab18586) , donkey anti-rabbit-Cy3 (Jackson Immuno Research, 711-166-152), donkey anti-rabbit-Alexa Fluor 647 (Jackson Immuno Research, 647 711-605-152), donkey anti-mouse-Cy5 AffiniPure (Jackson Immuno Research, 715-175-151), Hoechst (Sigma, B2261), Draq5 (Cell Signaling, 4085), DAPI (Roche, 10236276001), IWP2 (Tocris Bioscience, 3533).

Kumar N., Gurumurthy R.K., Prakash P.G., Kurian S.M., Wentland C., Brinkmann V., Mollenkopf H., Krammer T., Toussaint C., Saliba A., Biebl M., Jürgensen C., Wiedenmann B., Meyer T.F., & Chumduri C. (2021). Spatial organisation and homeostasis of epithelial lineages at the gastroesophageal junction is regulated by the divergent Wnt mucosal microenvironment.

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