Aldefluor
ALDEFLUOR is a flow cytometry-based reagent system designed to identify and isolate stem and progenitor cells based on the activity of the enzyme aldehyde dehydrogenase (ALDH). The ALDEFLUOR reagent is a fluorescent substrate for ALDH, allowing for the detection and analysis of ALDH-bright cells, which are enriched for stem and progenitor cell populations.
Lab products found in correlation
3 protocols using aldefluor
ALDH Activity and CD133 Expression
Isolation and Analysis of Tumor-Initiating Cells
The sorting process was through FACSAria™ III (BD Biosciences, Franklin Lakes, NJ, USA) for concomitant isolation of the ALDH-positive and the -negative cells, according to the ALDEFLUOR staining result. The cells were collected in the serum-free, growth factor-supplemented DMEM/F12 medium. At least 20,000 cells were used in each group for immediate RNA preparation, followed by the qPCR as described previously.
Comprehensive Flow Cytometry Analysis
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