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4 protocols using polyvinyl acetate pvac

1

Quantification of Safrole Analyte

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Polyvinyl acetate (PVAc) with a molecular weight of 500,000 g/mol was supplied by Sigma–Aldrich. N,N–dimethylformamide (DMF) for dissolving PVAc was purchased from Merck (Germany). Safrole analyte with 96% purity, was supplied by the Indonesian National Police, Jakarta, Indonesia. All chemicals were used as received without further purification. The AT–Cut QCM sensors with gold electrodes and 10 MHz base resonant frequency were purchased from OpenQCM, Novaetech. R&D, Napoli, Italy.
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2

Polymer Composites Synthesis and Characterization

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Polyvinyl acetate (PVAc) and polypyrrole (PPy) were provided by Sigma–Aldrich (Madrid, Spain). Gallic acid (C6H2(OH)3CO2H) (GA) was purchased from Sigma–Aldrich (Madrid, Spain). Potassium chloride (KCl), disodium-hydrogen phosphate (Na2HPO4), potassium dihydrogen phosphate (KH2PO4), and sodium chloride (NaCl) were purchased from Panreac Applychem (Barcelona, Spain). CO2 with a minimum purity of 99.8% was supplied by Linde (Barcelona, Spain).
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3

Synthesis and Characterization of Polymer Composites

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Poly(D,L-lactic-co-glycolic acid) (PLGA, MW 50-75 kg mol -1 ), poly(vinyl acetate) (PVAc, MW 113 kg mol -1 ), poly[(mphenylenevinylene)-alt-(2,5-dihexyloxy-p-phenylenevinylene)] (PMPDHPV), tetrahydrofuran (THF), chloroform, and N,Ndimethylformamide (DMF) were purchased from Sigma Aldrich. Methoxy-polyethylene glycol (PEG)-rhodamine (MW 5 kg mol -1 ) was purchased from Creative PEG Works.
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4

Biomaterial-based 3D Cell Culture Conditions

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Dulbecco Modified Eagle Medium, alpha-MEM, penicillin/ streptomycin, DPBS were obtained from Lonza. Ascorbic acid 2-phosphate and glycerol 2-phosphate disodium salt hydrate were purchased from Sigma. Fetal bovine serum and M199 medium was obtained from Gibco. ECGS/H were from Promocell. VEGF, TGFb 3 were from Peprotech. Poly(lactide-co-glycolide) (PLGA) (50 : 50 Resomer RG 502, RG 505, RG 504, and 85 : 15 PLGA ester terminated M w 50 000-75 000) and tween 20, biotin, chitosan (CS) (from crab shells), polyethylenimine (PEI) (average M w = 800 Da), poly(L-lactic acid) (PLLA) (viscosity B1.0 dL g À1 ) and poly(vinyl acetate) (PVAc) (average M w = 140 kDa), palmitic acid N-hydroxysuccinimide ester (NHS-palmitic acid) and avidin were from Sigma. All other reagents were from Sigma and used without further purification. Analytical grade chloroform, glacial acetic acid, dichloromethane (DCM) were from Thermo Fisher Scientific, UK. Emulsions were generated using a Branson sonifier 250 at 40% max amplification. 1 H nuclear magnetic resonance spectra were recorded on a Bruker AVA500 spectrometer (500 MHz respectively) at 298 K in deuterated solvents.
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