Anti pgc 1α antibody

Manufactured by Cell Signaling Technology

Sourced in United States

The Anti-PGC-1α antibody is a primary antibody that specifically binds to and recognizes the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein. PGC-1α is a transcriptional coactivator that plays a key role in the regulation of cellular energy metabolism.

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Lab products found in correlation

Anti acetylated lysine antibody, by Cell Signaling Technology (1 mentions) 3 3 diaminobenzidine dab staining, by Zhongshan Biotechnology (1 mentions) Anti gapdh antibody, by Abcam (1 mentions) β tubulin, by Cell Signaling Technology (1 mentions) Reverse transcription system, by Promega (1 mentions) Trizol reagent, by Promega (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Rapamycin, by Merck Group (1 mentions) Fenofibrate, by Merck Group (1 mentions) Anti cpt1b, by Abcam (1 mentions) Rabbit anti dsg2, by Abcam (1 mentions) Anti gapdh, by Abcam (1 mentions) Immobilon western chemiluminescent hrp substrate, by Merck Group (1 mentions) Anti pparα, by Abcam (1 mentions) Horseradish peroxidase conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions)

Spelling variants of this product

PGC-1α (72 citations) Anti-PGC-1α (22 citations)

4 protocols using anti pgc 1α antibody

PGC-1α Acetylation Analysis

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Cells were lysed with RIPA buffer containing 50mM Tris, pH 8.0, 150mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, supplemented with protease and phosphatase inhibitors and with 5mM nicotinamide and 1μM Trichostatin A to inhibit deacetylase activity. Lysates were incubated with anti-PGC-1α antibody (Cell Signaling, Danvers, MA) overnight at 4°C and the precipitated materials were used for western blot analysis using anti-acetylated lysine antibody (Cell Signaling, Danvers, MA) or with anti-PGC-1α antibody.

Hong Q., Zhang L., Das B., Li Z., Liu B., Cai G., Chen X., Chuang P.Y., He J.C, & Lee K. (2018). Increased podocyte Sirtuin-1 function attenuates diabetic kidney injury. Kidney international, 93(6), 1330-1343.

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Immunohistochemical Analysis of PGC-1α, SIRT3, and SOD2

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Immunohistochemistry staining was performed as previously described56 (link). The cardiac tissue was fixed with 10% formalin and embedded in paraffin. Anti-PGC-1α antibody (Cell Signaling Technology, MA, USA, 1:100 dilution), anti-SIRT3 antibody and anti-SOD2 antibody (Santa Cruz, CA, USA, 1:100 dilution) were used as the primary antibody. The positive area was detected by 3,3′-Diaminobenzidine (DAB) staining (Zhongshan biotechnology, Beijing, China). IgG antibody was used as a negative control. The images were photographed at 200× magnification (Olympus BX-63, JAPAN).

Yu L., Gong B., Duan W., Fan C., Zhang J., Li Z., Xue X., Xu Y., Meng D., Li B., Zhang M., Bin Z.h.a.n.g., Jin Z., Yu S., Yang Y, & Wang H. (2017). Melatonin ameliorates myocardial ischemia/reperfusion injury in type 1 diabetic rats by preserving mitochondrial function: role of AMPK-PGC-1α-SIRT3 signaling. Scientific Reports, 7, 41337.

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Quantification of PGC-1α Protein Levels

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Anti-PGC-1α antibodies were obtained from Cell Signaling Technology used for Western blot analysis. Anti-GAPDH antibody (Abcam) and β-tubulin (Cell Signaling Technology) were used as loading control. Signal intensities of PGC-1α protein bands were quantified using ImageJ (http://rsbweb.nih.gov/ij/) and normalized to β-tubulin signal intensities.

Taguchi A., Delgado O., Çeliktaş M., Katayama H., Wang H., Gazdar A.F, & Hanash S.M. (2014). Proteomic signatures associated with p53 mutational status in lung adenocarcinoma. Proteomics, 14(0), 2750-2759.

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Evaluating Metabolic Regulators in Cell Signaling

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Rapamycin, DMSO and fenofibrate were purchased from Sigma–Aldrich (St. Louis, MO, USA). Rabbit anti-phospho-mTOR (Ser2448), anti-mTOR, anti-phospho-4EBP1 (Thr37/46), anti-4EBP1, anti-PGC1α antibodies and mouse monoclonal anti-β-actin were from Cell Signaling Technology (Beverly, MA, USA). Rabbit anti-DSG2, anti-PPARα, anti-CPT1b, anti-ACADL, anti-GAPDH antibodies were from Abcam Inc. (Cambridge, MA, USA). Horseradish peroxidase-conjugated, donkey anti-rabbit IgG and donkey anti-mouse IgG were from Jackson ImmunoResearch (West Grove, PA, USA). Immobilon western chemiluminescent HRP substrate was purchased from Millipore (Temecula, CA, USA). Trizol reagent and the reverse transcription (RT) system were purchased from Promega Inc. (Madison, WI, USA).

Lin Y., Liu R., Huang Y., Yang Z., Xian J., Huang J., Qiu Z., Lin X., Zhang M., Chen H., Wang H., Huang J, & Xu G. (2022). Reactivation of PPARα alleviates myocardial lipid accumulation and cardiac dysfunction by improving fatty acid β-oxidation in Dsg2-deficient arrhythmogenic cardiomyopathy. Acta Pharmaceutica Sinica. B, 13(1), 192-203.

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