The effects of D-tagatose on S. mutans GS-5 biofilm formation were examined by SEM. Sterile plastic discs (Cell Desk, 13.5 mm in diameter; Sumitomo Bakelite Co., Ltd., Tokyo, Japan) were set in 24 well microtitre plates. The 48 h S. mutans GS-5 culture (30 µl) was mixed with 1 ml BHI containing 1% sucrose with or without xylitol (1 or 4%) or D-tagatose (1 or 4%) and 1 ml transferred into a well. Following 72 h anaerobic incubation, the biofilms formed on the discs were fixed with 2.5% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.4). Following fixation, they were dehydrated in a graded ethanol series and dried in a Hitachi PCP-2 critical point drying apparatus (Hitachi, Ltd., Tokyo, Japan). The discs were coated with platinum/palladium in a Hitachi E-102 sputter coater (Hitachi, Ltd.) and examined with a JEOL JCM-6000 scanning electron microscope (JEOL, Ltd., Tokyo, Japan). Biofilm areas were measured as described by Somayaji et al (24 (link)) using the auto-selection tool based on colour in Photoshop CS6 (Adobe Systems, Inc., San Jose, CA, USA), and the ratios to the total observation field were calculated. At least five randomly selected fields were examined.
Jcm 6000 scanning electron microscope
Manufactured by JEOL
Sourced in Japan
The JCM-6000 is a scanning electron microscope (SEM) manufactured by JEOL. It is a versatile instrument designed for high-resolution imaging and analysis of a wide range of materials. The JCM-6000 utilizes an electron beam to scan the surface of a sample, generating detailed images with high magnification and resolution. The core function of the JCM-6000 is to provide users with the ability to observe and analyze the microstructural and compositional characteristics of their samples.
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Lab products found in correlation
4 protocols using jcm 6000 scanning electron microscope
1
Examining D-tagatose Effects on S. mutans Biofilm
2
Diagnosing Hepatitis B-related Glomerulonephritis
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The diagnostic criteria used for CGN and HBV-GN were in accordance with the 2002 Kidney Disease Outcome Quality Initiative (K/DOQI), edited by the National Kidney Foundation (NKF) [14 (link)]. The diagnosis of HBV-GN was confirmed by pathology. Frozen slices from biopsies of the 54 HBV-GN patients were kept in a low-temperature freezer. Monoclonal goat-anti-human HBsAg and HBcAg antibodies were purchased from Dako (Denmark), and immunohistochemical staining for HBsAg and HBcAg in renal biopsies was used to confirm the diagnosis. For HBV-GN patients with undetectable HBsAg or HBcAg in the kidney tissue, HBV was detected using the JCM-6000 scanning electron microscope from Jeol, Ltd. (Japan) [15 (link)].
3
Analyzing Oral Biofilm Resistance to Washing
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Human oral biofilms were formed on 13.5-mm sterile plastic discs using the samples from #4 to #7. The discs were incubated in 0.5 ml BHI containing 1 % sucrose and 20 % human saliva. After a 72 h anaerobic cultivation, the discs were removed and washed once with saline. The biofilms on the discs were further washed with 0.5 ml saline, 10 mM citrate (pH3.5), or 0.5 M L-arginine (pH3.5) by shaking for 30 min at 37 °C. The discs were then washed again with 0.5 ml PBS (pH7.4), and the biofilms on the discs were fixed with 2 % glutaraldehyde in 0.1 M cacodylate buffer (pH7.2). After fixation, the biofilms were dehydrated in a graded ethanol series and dried in a Hitachi PCP-2 critical point drying apparatus. The discs were coated with platinum/palladium in a Hitachi E-102 sputter coater and examined with a JEOL JCM-6000 scanning electron microscope. The biofilm area retained after washing with each test reagent was measured using the color auto-selection tool in Photoshop CS6. The pixel value ratios of the biofilm area to total observation area were calculated from five randomly selected fields at 100x magnification.
4
Ligament Evaluation via Microscopy
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Ligaments were initially assessed with a Leitz MZ10 (Leitz) stereo microscope to assess their general condition. As sample 15 was of inadequate quantity for histological analysis, it was dehydrated in ethanol, gold coated, and viewed in secondary electron mode with a JCM6000 scanning electron microscope (JEOL). The section of sample 15 that was examined via SEM was taken from the junction of the intra-articular longitudinal fibers and the extra-articular woven fibers. The remnant was scanned, and a cross section through the woven extra-articular fibers was evaluated.
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