Easysep cd4 t cells enrichment kit

Leukapheresis samples were collected from HIV-infected individuals receiving suppressive antiretroviral therapy (ART). CD4+ T cells were isolated using the EasySep CD4+ T cells Enrichment Kit or a custom resting CD4+ T cells isolation kit (StemCell Technologies) as previously described (44, 46). The purified CD4+ T cells were plated at a density of 2x10⁶ cells and treated with indicated compounds. For the HIV gag RNA measurements in resting CD4+ T cells from ART-suppressed individuals, 3–5 replicates of 2–4 million resting CD4+ T cells per replicate were treated with compounds either for 24 or 48 hours. Cells were harvested, total RNA isolated and Gag HIV RNA measured as previously described after normalized with TBP internal control (Gay et al., 2020 (link)).
All participants provided informed consent and the study was approved by the UNC Institutional Review Board. The patient information, including age, gender, CD4+ T cell count, ART regimen and time under ART, have been included in Table 1.

Peripheral blood or leukapheresis samples were collected from HIV-positive individuals receiving suppressive ART for at least 3 years with undetectable viral loads. CD4⁺ T cells were isolated using the EasySep CD4⁺ T cells Enrichment Kit or a custom rCD4⁺ T cells isolation kit (StemCell Technologies).¹¹ (link)^,36 (link)^,37 (link) The purified CD4⁺ T cells were plated at a density of 1 × 10⁶ cells with or without HA15 treatment. For the HIV DNA measurements in rCD4⁺ T cells from ART-suppressed individuals, 3–4 replicates of 2–5 million rCD4⁺ T cells per replicate were treated with compounds. Total DNA was isolated from harvested cells. Then, gag HIV env DNA was measured.¹⁷ (link) All the participants were provided informed consent, and the study was approved by the UC-Davis and UNC-Chapel Hill Institutional Review Boards.