To record cilia motility, zebrafish embryos were embedded in 2% agarose (with 0.0175% Tricaine for 24 hpf embryos) on 50 mm glass-bottom dishes. Ciliary motility was viewed with a 63X water-dipping objective on an upright Zeiss Axioplan2 microscope equipped with a Hamamatsu ORCA-Flash4.0 V2 C11440-22CU camera. Processing of videos was performed with ImageJ 1.44d (Schneider et al., 2012 (link)).
Orca flash4.0 v2 c11440 22cu camera
Manufactured by Hamamatsu Photonics
The ORCA-Flash4.0 V2 C11440-22CU is a scientific-grade CMOS camera developed by Hamamatsu Photonics. It features a 4.0-megapixel sensor with global shutter and a high-speed readout of up to 100 frames per second. The camera is designed for a wide range of scientific and industrial applications that require high-resolution, high-speed image capture.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using orca flash4.0 v2 c11440 22cu camera
1
Zebrafish Ciliary Motility Imaging
2
Zebrafish Ciliary Motility Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To record cilia motility, zebrafish embryos were embedded in 2% agarose (with 0.0175% Tricaine for 24 hpf embryos) on 50 mm glass-bottom dishes. Ciliary motility was viewed with a 63X water-dipping objective on an upright Zeiss Axioplan2 microscope equipped with a Hamamatsu ORCA-Flash4.0 V2 C11440-22CU camera. Processing of videos was performed with ImageJ 1.44d (Schneider et al., 2012 (link)).
3
High-Speed Imaging of Auricle Cilia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All images were adjusted for brightness and contrast using Fiji (42) . High speed imaging of auricle cilia was captured using an ORCA-Flash4.0 V2 C11440-22CU camera from Hamamatsu mounted on a Zeiss Axiovert 200 microscope, as previously described (12) (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!