Whole blood was obtained from all chemically immobilized individuals by femoral venipuncture and collected into K3 EDTA S-Monovette collection tubes (Sarstedt). 1 ml of whole blood was cryopreserved in liquid nitrogen, ~50 μl was used for hematology, and the remainder was fractionated by centrifugation using a ficoll gradient. Fibroblasts were cultured from small biopsy punches of ear tissue that were stored in RPMI supplemented with 20% FBS and 10% DMSO. To maximize viability, these samples were frozen in steps by first storing in styrofoam at −20°C overnight, then transferring to liquid nitrogen.
We also measured hemoglobin concentrations using an AimStrip 78200 digital hemoglobin meter. We loaded 10 μl of venous blood into provided test strips and recorded hemoglobin concentrations (g/dl) using the digital meter. We report measurements from a total of 92 wild geladas sampled from 2017–2020.
We also measured hemoglobin concentrations using an AimStrip 78200 digital hemoglobin meter. We loaded 10 μl of venous blood into provided test strips and recorded hemoglobin concentrations (g/dl) using the digital meter. We report measurements from a total of 92 wild geladas sampled from 2017–2020.