Cell surface markers of ASCs and SHED were analyzed by flow cytometry. In detail, cells were identified as CD105, CD44, CD73, and CD90 positive cells and negative for CD45 expression. Standard labelling protocol was performed with the following antibodies fluorochrome-conjugated and isotypic controls: human CD105 PE (Invitrogen, Camarillo, CA, USA), CD73 FITC (kindly provided by Prof. Malavasi, University of Turin), CD44 FITC, CD45 PerCP, IgG1 PE and IgG2a PerCP (Miltenyi Biotech, Bergisch Gladbach, Germany), and IgG1 FITC-conjugated (Immunostep). As further control, unstained cells were also examined. About 105 events/sample were used for capture with CellQuest software. Data were analyzed with FlowLogic software (Miltenyi Biotech).
Igg1 pe and igg2a percp
Manufactured by Miltenyi Biotec
Sourced in Germany
IgG1 PE and IgG2a PerCP are fluorochrome-conjugated antibodies used in flow cytometry applications. IgG1 PE is an immunoglobulin G1 antibody conjugated to the fluorescent dye phycoerythrin, and IgG2a PerCP is an immunoglobulin G2a antibody conjugated to the peridinin-chlorophyll protein complex. These reagents can be used to detect and quantify target cells in a sample.
Automatically generated - may contain errors
2 protocols using igg1 pe and igg2a percp
1
Flow Cytometry of ASCs and SHED
2
Characterization of Mesenchymal Stem Cells
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The expression of typical MSCs markers was analyzed by flow cytometry. In detail, cells were identified as CD105-, CD44-, CD73- and CD90-positive, or CD45-, and CD3-negative.
Standard labeling protocol was performed with the following fluorochrome-conjugated antibodies and isotypic controls: human CD105 PE (Invitrogen, Camarillo, CA, USA), CD73 FITC (kindly provided by Prof. Malavasi, University of Turin), CD44 FITC, CD45 PerCP, CD3 APC, IgG1 PE and IgG2a PerCP (Miltenyi Biotech, Bergisch Gladbach, Germany), and FITC-conjugated IgG1 (Immunostep). As a further control, unstained cells were also examined. About 105 events/sample were used for capture with CellQuest software. Data were analyzed with FlowLogic software (Miltenyi Biotech).
Standard labeling protocol was performed with the following fluorochrome-conjugated antibodies and isotypic controls: human CD105 PE (Invitrogen, Camarillo, CA, USA), CD73 FITC (kindly provided by Prof. Malavasi, University of Turin), CD44 FITC, CD45 PerCP, CD3 APC, IgG1 PE and IgG2a PerCP (Miltenyi Biotech, Bergisch Gladbach, Germany), and FITC-conjugated IgG1 (Immunostep). As a further control, unstained cells were also examined. About 105 events/sample were used for capture with CellQuest software. Data were analyzed with FlowLogic software (Miltenyi Biotech).
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