Bm lactate test strips

Manufactured by Roche

The BM-lactate test strips are a diagnostic tool used to measure the level of lactate in a patient's blood. The test strips provide a quick and reliable way to assess lactate concentrations, which can be important for monitoring certain medical conditions.

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Lab products found in correlation

Accutrend plus meter, by Roche (1 mentions) Tmre mitochondrial membrane potential kit, by Abcam (1 mentions) Bsa pbs, by Merck Group (1 mentions) Accutrend plus, by Roche (1 mentions) 2 nbdg, by Thermo Fisher Scientific (1 mentions)

3 protocols using bm lactate test strips

Glucose and Lactate Production in Cells

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After transfection, cells were trypsinized and seeded into 6-well plates to allow the cells to form a complete monolayer. Glucose production was measured by the Contour next blood glucose monitoring system (meter, test strips and control solution). The Contour next system is intended for the quantitative measurement of glucose (from 0.6 mmol/L to 33.3 mmol/L) using approximately 10 μL of the supernatant (1 drop). Lactate production was measured by the Accutrend BM-lactate test strips with the Accutrend Plus meter from Roche (Art. No 128633, Basel, Switzerland), using approximately 10 μL of the supernatant (1 drop). The measurement took place at 24 h, 48 h, and 72 h without changing the medium.

Michail A., Gkikas D., Stellas D., Kaltezioti V, & Politis P.K. (2023). Prox1 Suppresses the Proliferation of Breast Cancer Cells via Direct Inhibition of c-Myc Gene Expression. Cells, 12(14), 1869.

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Assessing Metabolic Alterations in Stem Cells

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To analyze the mitochondrial membrane potential and assess the lactate concentration, 3000 cells per 100 µL CSC medium supplemented with 10% FCS were seeded in a 0.1% gelatin-coated 96 well-plate and after adherence 10 and 20 mM metformin applied. After 72 h, the mitochondrial membrane potential was assessed using the TMRE Mitochondrial Membrane Potential Kit (Abcam, Cambridge, U.K.) according to the manufacturer’s instructions. Briefly, 20 µM FCCP was added to the respective control wells 10 min prior to TMRE staining with 400 nM TMRE for 25 min. The medium was discarded, the cells were washed with 100 µL 0.2% BSA/PBS (Sigma-Aldrich, Munich, Germany), 100 µL 0.2% BSA/PBS was added to the cells and the fluorescence read at Ex525/Em580. To measure the lactate concentration of the media of treated and untreated ECSCs, 25 µL of the discarded medium was applied on the Accutrend^® Plus using the BM-Lactate test strips (Roche, Mannheim, Deutschland).

Helweg L.P., Windmöller B.A., Burghardt L., Storm J., Förster C., Wethkamp N., Wilkens L., Kaltschmidt B., Banz-Jansen C, & Kaltschmidt C. (2022). The Diminishment of Novel Endometrial Carcinoma-Derived Stem-like Cells by Targeting Mitochondrial Bioenergetics and MYC. International Journal of Molecular Sciences, 23(5), 2426.

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ALDOA Overexpression Glucose Uptake

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The glucose uptake of ALDOA-overexpressing cells was measured by a fluorescent probe, 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose (2-NBDG), as described previously (Zou et al., 2005 (link)). Briefly, Mel2400-ALDOA and Mel2400-RFP cells were seeded in 6-well plates and allowed to stabilize overnight. The next day, the cells were washed with PBS and subsequently incubated with glucose-free culture medium with 50 uM of 2-NBDG (Molecular Probes) at 37°C for 30 minutes. The 2-NBDG uptake reaction was stopped by washing with cold PBS and maintained in PBS at 4°C. Flow cytometry analysis was performed within 30 min after the completion of 2-NBDG incubation. Cells without 2-NBDG incubation were used to determine the baseline glucose uptake levels. The glucose uptake rate was determined by the changes of fluorescent intensity between cells after 2-NBDG incubation and baseline. To measure the lactate production, Mel2400-ALDOA and Mel2400-RFP cells were seeded in 6-well plates at 6 × 10⁵ cells/well and cultured at 37 °C with 5% CO₂ for 48 hours. The lactic acid concentration of conditioned medium was determined by the Accutrend Lactate Analyzer using BM lactate test strips (Roche).

Cascone T., McKenzie J.A., Mbofung R.M., Punt S., Wang Z., Xu C., Williams L.J., Wang Z., Bristow C.A., Carugo A., Peoples M.D., Li L., Karpinets T., Huang L., Malu S., Creasy C., Leahey S.E., Chen J., Chen Y., Pelicano H., Bernatchez C., Vashisht Gopal Y.N., Heffernan T.P., Hu J., Wang J., Amaria R.N., Garraway L.A., Huang P., Yang P., Wistuba I.I., Woodman S.E., Roszik J., Davis R.E., Davies M.A., Heymach J.V., Hwu P, & Peng W. (2018). Increased Tumor Glycolysis Characterizes Immune Resistance to Adoptive T cell Therapy. Cell metabolism, 27(5), 977-987.e4.

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