Serum-soluble CXCL16 concentration was measured with the human CXCL16 Quantikine ELISA Kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions. Absorbance was measured by a Benchmark Microplate reader (Bio-Rad, Hercules, CA).
Total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), and triglycerides (TG) were assessed by routine methods using a fully automatic biochemical analyzer (Hitachi 7600-020, Hitachi, Tokyo, Japan). Serum high sensitivity C-reactive protein (hs-CRP) was measured by immunoturbidimetric assay with the automatic biochemical analyzer. Plasma fibrinogen (Fg) was measured by the Von Clauss coagulative method (Fibri-Prest Automate, Diagnostica Stago, Asnieres, France).
Total cholesterol (TC), low-density lipoprotein (LDL), high-density lipoprotein (HDL), and triglycerides (TG) were assessed by routine methods using a fully automatic biochemical analyzer (Hitachi 7600-020, Hitachi, Tokyo, Japan). Serum high sensitivity C-reactive protein (hs-CRP) was measured by immunoturbidimetric assay with the automatic biochemical analyzer. Plasma fibrinogen (Fg) was measured by the Von Clauss coagulative method (Fibri-Prest Automate, Diagnostica Stago, Asnieres, France).