Leiboviz s l 15 medium

The human cell lines SW620, SW480, HCT116, DLD1, RKO, HUVEC and THP-1 and the mouse cell lines CT26, CMT93, MC38, RAW264.7 and C166 were preserved in the Department of Pathology, Southern Medical University, China. SW620 and SW480 cells were cultured in Leiboviz’s L-15 medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco). HCT116 cells were cultured in McCoy’s 5A medium (Gibco) with 10% FBS. RKO and HUVEC cells were cultured in F-12 K medium (Gibco) with 10% FBS. DLD1, THP-1, CT26 and MC38 cells were cultured in RPMI 1640 medium (Gibco) with 10% FBS (Gibco). CMT93, RAW264.7 and C166 were cultured in Dulbecco's Modified Eagle's Medium (DMEM) (Gibco) supplemented with 10% FBS.

The cell lines used in this study, including the human CRC cell lines SW480, CaCo2, HCT15, HCT116, SW620, HT29, LoVo, RKO, and mouse CRC cell line MC38, were purchased from the American Type Culture Collection (ATCC) and were stored in the Department of Pathology of Southern Medical University. SW480 and SW620 cells were cultured in Leiboviz’s L-15 medium (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco). CaCo2, LoVo, HT29, and MC38 cells were cultured in DMEM (Gibco) supplemented with 10% FBS. HCT15 and RKO cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% fetal bovine serum (FBS). HCT116 cells were cultured in McCoy’s 5A medium (Gibco) supplemented with 10% fetal bovine serum (FBS). All cells were cultured at 37 °C in a 5% CO₂ atmosphere.