Intact parathyroid hormone i pth immunoradiometric assay

We collected peripheral blood samples at the beginning of hemodialysis during the first session of the week. Hemoglobin level, white blood cell counts, blood biochemistry including blood urea nitrogen (BUN), creatinine, albumin, total cholesterol, triglyceride, calcium, and phosphate were measured. Kt/V and normalized protein catabolic rate (nPCR) were calculated to represent dialysis adequacy and dietary protein intake; high sensitivity C-reactive protein (hsCRP) nephelometry (from Siemens) and intact-parathyroid hormone (i-PTH) immunoradiometric assay (from CISBio International) were assessed at the same time. Peripheral blood mononuclear cells (PBMCs) were isolated and submitted for flow cytometry analysis. LRG1 was measured by ELISA (from RayBio Human assay). The plasma levels of inflammatory cytokines, TNF-α and IL-6, were assayed with human TNF-α Quantikine ELISA kit and human IL-6 Quantikine HS ELISA kit and, respectively (from R&D systems).

Blood samples were collected before the start of hemodialysis sessions in the middle of week. In addition to hematological and biochemical tests, peripheral blood mononuclear cells (PBMC) were sampled. Intact-parathyroid hormone (i-PTH) immunoradiometric assay (Cisbio) and high sensitive C-reactive protein (hs-CRP) nephelometry (Siemens) were also tested. By history taking and detailed chart reviews, baseline co-morbidities and clinical laboratory data were recorded. HCMV-specific IgG titer was measured by ELISA (Roche Elecsys assay) at Far Eastern Memorial Hospital. Plasma level of inflammatory cytokines were measured using the human IL-6 Quantikine HS ELISA kit and human TNFα Quantikine ELISA Kit from R&D systems.