The largest database of trusted experimental protocols

Rabbit anti tlr3

Manufactured by Abcam
Sourced in United Kingdom

Rabbit anti-TLR3 is a primary antibody that recognizes the Toll-like receptor 3 (TLR3) protein. TLR3 is a member of the Toll-like receptor family and plays a role in the innate immune response to double-stranded RNA. This antibody can be used for various research applications, such as western blotting, immunohistochemistry, and flow cytometry.

Automatically generated - may contain errors

2 protocols using rabbit anti tlr3

1

Recombinant Interferons and Antibodies for EV71 Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant human IFN-α2b was purchased from Sinobioway Medicine (Tianjin, China). Recombinant human IFN-λ1 was purchased from Novoprotein (Shanghai, China). Recombinant murine IFN-λ2 was purchased from PeproTech (Rocky Hill, NJ, USA). Recombinant human IFN-γ (catalog no. 285-IF) was obtained from R&D Systems (Minneapolis, MN, USA). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, CA, USA). Rabbit anti-EV71 VP1 antibody was obtained from Abnova (Taipei, Taiwan). Rabbit anti-TLR3 and anti-IL-28+IL-29 antibodies were purchased from Abcam (Cambridge, United Kingdom). Rabbit anti-IRF1 antibody was obtained from Cell Signaling Technology (Beverly, MA, USA). Rabbit antibodies to β-actin and EV71 3C were purchased from ABclonal Technology (Wuhan, China). Mouse anti-glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH) was purchased from Proteintech Group (Rosemont, IL, USA). Mouse monoclonal anti-dsRNA J2 antibody was obtained from Scicons (Budapest, Hungary). Rabbit anti-dsRNA (J2) was purchased from Absolute Antibody (Wilton, United Kingdom). Mouse anti-MUC2, anti-lysozyme C, and anti-ChrA were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Poly(I·C) was obtained from InvivoGen (San Diego, CA, USA).
+ Open protocol
+ Expand
2

Immunohistochemistry and Molecular Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunohistochemistry was performed as in Yi et al. using rabbit anti-TLR3 (1:100 dilution, Abcam, Cambridge, MA), mouse anti-rhodopsin (1:300 dilution, Millipore, Billerica, MA), goat anti-rabbit Alexa 488 (1:600, Santa Cruz, Dallas, TX), and goat anti-mouse Alexa 546 (1:600, Santa Cruz, Dallas, TX) antibodies (Yi et al., 2012 (link)). QPCR analysis was performed using primers for IRF3, IL-6, Stat3, and TLR3 (Table 1). Western blotting was performed using the following antibodies: phosphorylated Stat3 (1:200 dilution, Cell Signaling, Danvers, MA), total Stat3 (1:200 dilution, Cell Signaling), and β-actin (1:8000 dilution, Sigma, St. Louis, MO) as in Yi et al (Yi et al., 2012 (link)).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!