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Zoletil mixture

Manufactured by Virbac
Sourced in France

Zoletil mixture is a laboratory product manufactured by Virbac. It is a combination of two active substances: tiletamine and zolazepam. The core function of this product is to provide a general anesthetic and sedative effect for the purpose of animal research and testing.

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6 protocols using zoletil mixture

1

Rat Model of Reflux Esophagitis Induction

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Sham and the other groups received a sham surgery or RE induction surgery 1 h after the last pretreatment, respectively [22 (link)]. Rats were anesthetized with Zoletil mixture™ (25 mg/kg, i.p.; Virbac, France). For RE induction, the abdomen was opened by a 2 cm median incision. The transitional region between the forestomach and corpus and the pylorus portion were ligated with a 2–0 silk thread (B. Braun Surgical SA, Spain) (Figure 1). A 1 cm longitudinal cardiomyotomy was performed across the gastroesophageal junction. The incised region was immediately sutured. For the sham, the abdomen was opened in the same manner as the RE induction and closed immediately without ligation and cardiomyotomy. All animals were sacrificed after collection of 1 ml of blood from the orbital plexus at 8 h after surgery and samples of esophagus and stomach tissue; gastric contents were collected.
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2

Niacinamide Tissue Depot Study

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Pigs (70–90 kg) were anesthetized using Zoletil mixture (1 mg/10 kg; Virbac, Denmark) with intravenous supplement of propolipid (Fresenius Kabi, Sweden). Pigs were placed on their side and injected in the neck region with 50 μl of phosphate buffer (10 mM; pH 7.4) supplemented with niacinamide (170 mM; adjusted to isotonic with 85 mM NaCl) or 1-methyl-niacinamide (170 mM). Tissue samples measuring ~2 x 2 cm were excised at 0 min (acute), 2, 5, 15 or 60 min after injection and immediately frozen in 2-methyl-butane. To evaluate whether niacinamide and 1-methyl-niacinamide were entirely confined within the excised tissue, 1 cm thick sections along the four edges of the 0 min (acute) sample were also excised. Following euthanasia by overdose of pentobarbital (Lundbeck, Denmark), tissue samples measuring ~10 x 10 cm were excised and injected to obtain ex vivo 0 min (acute) and 60 min depots.
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3

Balb/c Mice Anesthesia Protocol

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The laboratory animals were treated according to the rules of Saratov State Medical University (Ethics Committee Protocol No. 7, dated 2 February 2021) and the Geneva Convention of 1985 (International Guiding Principles for Biomedical Research Involving Animals). All experimental procedures were performed on white Balb/c male mice 6–8 weeks old (20–25 g weight) using general anesthesia (Zoletil mixture (40 mg per kg, 50 μ L, Virbac SA, Carros, France) and 2% Rometar (10 μ L and 10 mg per kg, Spofa, Czech Republic)) via intraperitoneal injection. At the end of the experiment, the animals were euthanized by an overdose of anesthesia.
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4

Balbc Mice Euthanasia Protocol

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All animals for this study were housed in the Animal Care Facility of Ogarev Mordovia State University under standard conditions with free access to food and water. All animal procedures were performed according to a protocol approved by the Institutional Animal Care and Use Committees of the Medical Institute of Ogarev Mordovia State University (Ethics Committee protocol # 50 from 20.05.2017). Balbc mice, mix population of 6–8 week-old, were euthanized with Zoletil mixture (40 mg kg-1, 50 μl, Virbac SA, Carros, France) and 2% Rometar (10 μl and 10 mg kg-1, Spofa, Czechia) via intraperitoneal injection.
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5

Microcapsule Intravenous Injection in Mice

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Experiments were performed on Balb mice 6–8 weeks old with an average weight of 20–25 g under general anesthesia (Zoletil mixture, 50 μ L, 40 mg/kg (Virbac SA, Carros, France) and 2% Rometar, 10 μ L, 10 mg/kg (Spofa, Czech Republic) via intraperitoneal injection). All experimental procedures were carried out according to the rules of Saratov State Medical University (Ethics Committee protocol no. 5, 29 December 2018). A microcapsule suspension (0.2 mL; 1, 2.7, and 5.5 μ m in size) was injected into the tail vein at a dosage of 10 7 microcapsules per mouse. After the experiment was completed, the animals were sacrificed using an overdose of anesthesia.
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6

Balb/c Mouse Anesthesia Protocol

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The experiments on animals were carried out at the Ogarev Mordovia State University. All in vivo and ex vivo procedures were treated according to the rules of the University Ethics Committee (Protocol No. 100, dated 29 November 2021, Saransk, Russia) and the Geneva Convention of 1985 (International Guiding Principles for Biomedical Research Involving Animals). The experiments were performed on Balb/c female mice 8 weeks old (25 g weight) using general anesthesia via intraperitoneal injection (Zoletil mixture (40 mg per kg, 50 μL, Virbac SA, Carros, France) and 2% Rometar (10 μL and 10 mg per kg, Spofa, Czech Republic)). The animals were euthanized by an overdose of anesthesia at the end of the experiments.
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