Pip3 mass elisa

Manufactured by Echelon Biosciences

Sourced in United States

The PIP3 Mass ELISA is a quantitative enzyme-linked immunosorbent assay used to measure the concentration of phosphatidylinositol (3,4,5)-trisphosphate (PIP3) in biological samples. The assay employs a competitive binding technique, where the PIP3 in the sample competes with a fixed amount of horseradish peroxidase (HRP)-labeled PIP3 for binding to anti-PIP3 antibodies coated on the microplate wells. The amount of HRP-labeled PIP3 bound to the antibodies is inversely proportional to the concentration of PIP3 in the sample, allowing for the quantification of PIP3 levels.

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Lab products found in correlation

Human insulin, by Merck Group (1 mentions) Sandwich elisa, by Cell Signaling Technology (1 mentions) Pi 3 4 p2 mass elisa, by Echelon Biosciences (1 mentions)

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PIP3 Mass ELISA Kit (10 citations)

3 protocols using pip3 mass elisa

Measuring PIP3 Levels via ELISA

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PIP3 Mass ELISA (Echelon) was performed according to the manufacturer's protocol. Values were normalized to total protein levels measured in a small portion of the total lysate obtained prior to lipid purification.

Goldsmith J.R., Spitofsky N., Zamani A., Hood R., Boggs A., Li X., Li M., Reiner E., Ayyaz A., Etwebi Z., Lu L., Rivera Guzman J., Bou-Dargham M.J., Cathoupolis T., Hakonarson H., Sun H., Wrana J.L., Gonzalez M.V, & Chen Y.H. (2020). TNFAIP8 controls murine intestinal stem cell homeostasis and regeneration by regulating microbiome-induced Akt signaling. Nature Communications, 11, 2591.

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Insulin Stimulation of 3T3-L1 Adipocytes

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3T3-L1 fibroblasts were seeded and differentiated in PLL coated 10 cm dishes or T-175 flasks. Mature adipocytes were stimulated with or without 10 nmol/L human insulin (Sigma-Aldrich) 30 minutes at 37°C. One confluent 10 cm dish per experimental condition was used for the Phospho-Insulin Receptor b (Tyr1150/1151) Sandwich ELISA (Cell Signaling Technology, Leiden, the Netherlands) and one confluent T-175 flask per experimental condition was used for the PIP3 Mass Elisa (Echelon Biosciences, Salt Lake City, UT, USA) were used according to the manufacturer’s protocol.

Oost L.J., Kurstjens S., Ma C., Hoenderop J.G., Tack C.J, & de Baaij J.H. (2022). Magnesium increases insulin-dependent glucose uptake in adipocytes. Frontiers in Endocrinology, 13, 986616.

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Quantifying Phosphoinositide Isoforms

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PIP isoforms were quantified from extracted lipids using PI(4,5)P2 Mass ELISA (K‐4500), PI(3,4)P2 Mass ELISA (K‐3800) and PIP3 Mass ELISA (K‐2500s) from Echelon Biosciences using the manufacturer’s instructions.

Maguire E., Menzies G.E., Phillips T., Sasner M., Williams H.M., Czubala M.A., Evans N., Cope E.L., Sims R., Howell G.R., Lloyd‐Evans E., Williams J., Allen N.D, & Taylor P.R. (2021). PIP2 depletion and altered endocytosis caused by expression of Alzheimer's disease‐protective variant PLCγ2 R522. The EMBO Journal, 40(17), e105603.

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