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Trypan blue t8154

Manufactured by Merck Group
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Sourced in Germany

Trypan Blue T8154 is a dye used in cell biology and other laboratory applications. It is a vital stain used to selectively color dead cells blue. The dye is excluded from living cells, allowing viable cells to remain unstained.

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Lab products found in correlation

Fura2 am, by PromoCell (2 mentions) Ascorbic acid a4403, by Merck Group (1 mentions) Capsazepine, by Cayman Chemical (1 mentions) Cisplatin s1166, by Selleck Chemicals (1 mentions) Sb202190, by Selleck Chemicals (1 mentions) Am251, by Cayman Chemical (1 mentions) Icilin, by Cayman Chemical (1 mentions) Win55 212 2, by Cayman Chemical (1 mentions) Z vad fmk001, by R&D Systems (1 mentions) Cis nerolidol, by Merck Group (1 mentions)

4 protocols using trypan blue t8154

1

Cell Culture and Patch-Clamp Assays

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Medium and supplements for cell culture were purchased from Biochrom AG (Berlin, Germany) or GIBCO Invitrogen (Karlsruhe, Germany). All reagents (e.g., Ascorbic acid A4403; Trypan Blue T8154), except those specified below, were purchased from Sigma-Aldrich (Deisenhofen, Germany). Capsazepine was purchased from Cayman Chemical Company (Ann Arbor, Mi, USA). The internal and external solutions for planar patch-clamping were provided by Nanion Company (Munich, Germany). Fura2/AM was purchased from PromoCell (Heidelberg, Germany).
Oronowicz J., Reinhard J., Reinach P.S., Ludwiczak S., Luo H., Omar Ba Salem M.H., Kraemer M.M., Biebermann H., Kakkassery V, & Mergler S. (2020). Ascorbate-induced oxidative stress mediates TRP channel activation and cytotoxicity in human etoposide-sensitive and -resistant retinoblastoma cells. Laboratory Investigation; a Journal of Technical Methods and Pathology, 101(1), 70-88.
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2

Small Molecule Screening Protocol

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5-FU (F6627), Wnt3a (H17001), LiCl (L9650), and Trypan blue (T8154) were purchased from Sigma-Aldrich. Cisplatin (S1166), Ralimetinib (S1494), Trametinib (S2673), Irinotecan (S2217), PRI-724 (S8262), and SB202190 (S1077) were purchased from SelleckChem.
Lepore Signorile M., Grossi V., Di Franco S., Forte G., Disciglio V., Fasano C., Sanese P., De Marco K., Susca F.C., Mangiapane L.R., Nicotra A., Di Carlo G., Dituri F., Giannelli G., Ingravallo G., Canettieri G., Stassi G, & Simone C. (2021). Pharmacological targeting of the novel β-catenin chromatin-associated kinase p38α in colorectal cancer stem cell tumorspheres and organoids. Cell Death & Disease, 12(4), 316.
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3

Cell Culture Reagents and Sourcing

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Cell culture medium and other cell culture supplements were purchased from Biochrom AG (Berlin, Germany) or GIBCO Invitrogen (Karlsruhe, Germany). All reagents (e.g., trypan blue T8154) were purchased from Sigma-Aldrich (Diesenhofen, Germany) unless otherwise specified. Recombinant Human β-NGF was purchased from Biogems company (Westlake Village, CA, USA). Icilin, AM251, and WIN55,212-2 were purchased from Cayman Chemical Company (Ann Arbor, MI, USA). For calcium imaging, the fluorescent dye fura-2/AM was purchased from PromoCell GmbH (Heidelberg, Germany).
Ludwiczak S., Reinhard J., Reinach P.S., Li A., Oronowicz J., Yousf A., Kakkassery V, & Mergler S. (2024). Joint CB1 and NGF Receptor Activation Suppresses TRPM8 Activation in Etoposide-Resistant Retinoblastoma Cells. International Journal of Molecular Sciences, 25(3), 1733.
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4

Nerolidol and Small Molecule Inhibitors

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cis-Nerolidol (from here written as nerolidol) was obtained from Sigma-Aldrich (St. Louis, MO, USA) (72180). Small molecular inhibitors: xestospongin C (XSC; 64950), dandrolene-Na (DRN; 14326), SB203508 (13067), H-89 (10010556), KH7 (13243), and L755507 (18629) were obtained from Cayman Chemicals. Z-Vad (FMK001) was obtained from R&D systems. All chemicals were dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich; D4540). MTT (M5655) and trypan blue (T8154) were obtained from Sigma-Aldrich.
Glumac M., Čikeš Čulić V., Marinović-Terzić I, & Radan M. (2023). Mechanism of cis-Nerolidol-Induced Bladder Carcinoma Cell Death. Cancers, 15(3), 981.
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