Cell-free supernatants from resting or stimulated Vγ9/Vδ2 T cell, DC, or monocyte cultures were collected after 3 d incubation. Supernatants from 50,000 polarized CD4+ T cells were collected after 24 h incubation with 10 ng/ml PMA and 1 μg/ml ionomycin. Supernatants from intestinal tissue cells were collected after 3 d in culture. Soluble cytokines were detected using conventional ELISA kits for IFN-γ and calprotectin (BioLegend); TNF-α, IL-10, IL-17, IL-22, and IL-23 (eBioscience); and IL-1β, IL-6, and IL-12p70 (R&D Systems). All samples were measured in duplicate on a Dynex MRX II reader.
Mrx 2 reader
Manufactured by Dynex
The MRX II reader is a versatile laboratory instrument designed for optical absorbance measurements. It is capable of performing a wide range of spectrophotometric analyses across multiple wavelengths. The core function of the MRX II reader is to accurately measure the absorbance of samples, which is a fundamental technique in various scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mrx 2 reader
1
Cytokine Profiling of Immune Cell Cultures
2
Cytokine Profiling of Immune Cells
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