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1 ethyl 3 3 dimethylaminopropyl carbodiimide hcl edc

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1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) is a chemical compound used as a crosslinking agent in various laboratory applications. Its primary function is to facilitate the formation of covalent bonds between carboxyl groups and primary amine groups, enabling the conjugation of molecules such as proteins, peptides, or other biomolecules.

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Lab products found in correlation

Cyclohexane, by Merck Group (4 mentions) N hydroxysuccinimide, by Merck Group (4 mentions) Heparin, by Merck Group (2 mentions) Phosphate buffered saline (pbs), by Merck Group (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Hexane, by Thermo Fisher Scientific (2 mentions) Ethanol, by Thermo Fisher Scientific (2 mentions) 1 4 dioxane, by Thermo Fisher Scientific (2 mentions) Deferoxamine mesylate, by Abcam (2 mentions) 2 n morpholino ethanesulfonic acid hydrate, by Merck Group (2 mentions) Gelatin type b, by Merck Group (2 mentions) Magplex microsphere, by DiaSorin (2 mentions) Xmap antibody coupling kit, by DiaSorin (2 mentions) 11 mercaptoundecanoic acid, by Merck Group (2 mentions) Potassium hexacyanoferrate 2 trihydrate fe cn 6 4, by Merck Group (2 mentions) Anhydrous ethanol solution, by Merck Group (2 mentions) 2 n morpholino ethanesulfonic acid mes buffer, by Merck Group (2 mentions) Amine peg2 biotin, by Thermo Fisher Scientific (2 mentions) Milli q water, by Merck Group (2 mentions)

5 protocols using 1 ethyl 3 3 dimethylaminopropyl carbodiimide hcl edc

1

Nickle Foam Functionalization Protocol

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Nickle foam was purchased from Taili (Suzhou, China). Poly (methyl methacrylate) (PMMA), (2-(Nmorpholino) ethanesulfonic acid) hydrate (MES), N-hydroxy-succinimide (NHS), heparin, bovine serum albumin, phosphate buffered saline, and cyclohexane were purchased from Sigma (St. Louis MO, USA). And 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) was purchased from Thermal Scientific (Rockford, USA).
Yao Q., Liu Y, & Sun H. (2018). Heparin-Dopamine Functionalized Graphene Foam for Sustained Release of Bone Morphogenetic Protein-2. Journal of tissue engineering and regenerative medicine, 12(6), 1519-1529.
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2

Bovine Gelatin-Based Biopolymer Synthesis

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Gelatin type B (from bovine skin, 225 Bloom), (2-(N-morpholino) ethanesulfonic acid) hydrate (MES), N-hydroxy-succinimide (NHS), and cyclohexane were purchased from Sigma (St. Louis MO, United States). Deferoxamine mesylate was purchased from Abcam (Abcam, Cambridge, MA, United States). Ethanol, 1, 4-dioxane, and hexane were purchased from Fisher Scientific (New Jersey, United States). 1-Ethyl-3-(3-(dimethylamino)propyl) carbodiimide HCl (EDC) was purchased from Thermal Scientific (Rockford, United States).
Yao Q., Liu Y., Tao J., Baumgarten K.M, & Sun H. (2016). Hypoxia-Mimicking Nanofibrous Scaffolds Promote Endogenous Bone Regeneration. ACS applied materials & interfaces, 8(47), 32450-32459.
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3

Purification and Coupling of G Proteins

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An amount of 50 μg of purified G protein was coupled to 1.25 × 107 MagPlex microspheres (Luminex Corporation, Austin, TX, USA) using an xMAP Antibody Coupling Kit (Luminex Corporation). For the microsphere activation, 1.25 × 107 of the stock microspheres was transferred to the recommended microcentrifuge tubes. The liquid was removed using a 1.5 mL tube magnetic separator (Luminex Corporation). The microspheres were washed once in dH2O. The washed microspheres were resuspended in 80 μL of 0.1 M sodium phosphate (monobasic, pH 6.2) by vortex and sonication for approximately 20 s. Subsequently, the microspheres were incubated in an activation buffer containing 5 mg/mL 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) (Thermo Fisher Scientific) and 5 mg/mL N-hydroxysulfosuccinimide (S-NHS) (Luminex Corporation) for 20 min at 24 °C with shaking in the dark. The liquid was removed, and the purified G proteins were added. The microspheres and antigens were incubated for 2 h at 24 °C with shaking in the dark. The microspheres were washed twice with PBSA (PBS, 1% BSA) and resuspended in a 600 μL microsphere storage buffer (Luminex Corporation).
Li Y., Li R., Wang M., Liu Y., Yin Y., Zai X., Song X., Chen Y., Xu J, & Chen W. (2020). Fc-Based Recombinant Henipavirus Vaccines Elicit Broad Neutralizing Antibody Responses in Mice. Viruses, 12(4), 480.
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4

Electrochemical Biosensor Fabrication

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30% hydrogen peroxide (H2O2), potassium nitrate (KNO3), ethanolamine, amine-PEG2-biotin, 1-ethyl-3-(3-(dimethylamino)propyl)-carbodiimide HCl (EDC), and potassium ferricyanide (Fe-(cn)63–) were purchased from Thermofisher Scientific (Fairlawn, NJ, USA). Anhydrous ethanol solution, 2-(N-morpholino)ethanesulfonic acid (MES) buffer, sulfo-N-hydroxsulfosuccinimide (NHS), lipoic acid, 11-mercaptoundecanoic acid (MUA), and potassium hexacyanoferrate(II) trihydrate (Fe(CN)64–) were purchased from Sigma-Aldrich (St. Louis, MO). 6-(Ferrocenyl)hexanethiol (Fc-(CH)6SH) was purchased from Santa Cruz Biotech (Dallas, TX). Sodium hydroxide (NaOH) was purchased from JT Baker (TX, USA). All solutions were prepared in ultrapure Milli-Q water (18.2 MΩ cm, MilliPore, MA, USA). Whatman 1 chromatography paper was purchased from GE healthcare sciences (UK). Scotch Heavy Duty Shipping packing tape (3M) was purchased from Office Max. Streptavidin (SA) microparticles were purchased from Life Technologies. High purity silver paint was purchased from SPI Supplies (West Chester, PA). Au microwires (99.99% pure, 25 μm diameter) were purchased from California Fine Wire Company (Grover Beach, CA). 0.05 M pH 6.0 MES buffer was made with MES, and pH was adjusted to 6.0 with NaOH.
Channon R.B., Yang Y., Feibelman K.M., Geiss B.J., Dandy D.S, & Henry C.S. (2018). Development of an Electrochemical Paper-Based Analytical Device for Trace Detection of Virus Particles. Analytical chemistry, 90(12), 7777-7783.
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5

Serological Profiling of Pneumococcal Antigens

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The presence of antibodies against S. pneumoniae was investigated using eight distinct pneumococcal proteins: pneumolysin [Ply], choline binding protein A [CbpA], pneumococcal surface protein A families 1 and 2 [PspA 1 and PspA2], pneumococcal choline binding protein A [PcpA], pneumococcal histidine triad protein D [PhtD], serine/threonine protein kinase [StkP-C, SP1732-3, a C-terminal fragment of StkP], and protein required for cell wall separation of group B streptococcus [PcsB-N, SP2216-1, a N-terminal fragment of PcsB]). Hydroxysulfosuccinimide (Sulfo-NHS) and 1-ethyl-3 (3dimethylamino-propyl) carbodiimide-HCl (EDC) were provided by Thermo Fisher Scientific, Rockford, IL, USA. R-phycoerthyryn (R-PE)-conjugated AffiniPure goat anti-human IgG, Fc γ Fragment specific was obtained from Jackson ImmunoResearch Laboratories Inc. (Westgrove, PA, USA).
Andrade D.C., Borges I.C., Ivaska L., Peltola V., Meinke A., Barral A., Käyhty H., Ruuskanen O, & Nascimento-Carvalho C.M. (2016). Serological diagnosis of pneumococcal infection in children with pneumonia using protein antigens: A study of cut-offs with positive and negative controls. Journal of immunological methods, 433.
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