M mlv reverse transcription enzyme

The superior right lobe was excised and total RNA was extracted using Tri Reagent solution (Invitrogen, CA, USA) from tissue homogenates. Following DNase treatment (Invitrogen, CA, USA), reverse transcription was performed using M-MLV reverse transcription enzyme (Invitrogen, CA, USA) and random primers (Invitrogen, CA, USA). Quantitative real-time PCR was performed with SYBR green (Applied Biosystem) using the following primers:
Mouse CCL2: forward 5′-CCAACTCTCACTGAAGCCAGCTCT-3′
reverse 5′-TCAGCACAGACCTCTCTCTTGAGC-3′
Mouse CCL7: forward 5′-GCTTCTGTGCCTGCTGCTCATA-3′
reverse 5′-CATAGCAGCATGTGGATGCATTG-3′
Mouse CCL20: forward 5′-CGACTGTTGCCTCTCGTACA-3′
reverse 5′-AGGAGGTTCACAGCCCTTTT-3′
Mouse G-CSF: forward 5′-ACTCAGGGAAGCCTTCGG-3′
reverse 5′-GCAAGTGAGGAAGATCCAGG-3′
Mouse M-CSF: forward 5′-GGTAGTGGTGGATGTTCCCA-3′
reverse 5′-CCAGGATGAGGACAGACAGG-3′
Mouse HPRT: forward 5′-AGGCCAGACTTTGTTGGATTTGAA-3′
reverse 5′-CAACTTGCGCTCATCTTAGGCTTT-3′
Gene expression was normalized relative to the housekeeping gene HPRT and expressed as a fold change relative to uninfected mice using the 2^−ΔΔCt formula.