Tissue lysates were collected in lysis buffer (20 × 10−3m Tris (pH 7.5), 150 × 10−3m NaCl, 1% Triton X‐100, and 1 × 10−3m PMSF). SDHA, SDHB, ubiquitin protein were immunoprecipitated using anti‐SDHA antibody (5839, CST), SDHB antibody (178423, Abcam), and Ubiquitin antibody (8017, Santa Cruz, City, CA), respectively. SDHA, SDHB, and ubiquitin were western blotted using anti‐SDHA antibody (390381, Santa Cruz), anti‐SDHB antibody (271548, Santa Cruz), and anti‐ubiquitin‐HRP (8017, Santa Cruz).
Anti sdha antibody
Manufactured by Santa Cruz Biotechnology
The Anti-SDHA antibody is a tool used in research applications to detect the presence and quantify the levels of the SDHA (Succinate Dehydrogenase Complex Subunit A) protein. SDHA is a key component of the mitochondrial respiratory complex II and plays a crucial role in cellular energy metabolism. This antibody can be utilized in various analytical techniques, such as Western blotting, immunohistochemistry, and immunocytochemistry, to support research into mitochondrial function and energy production processes.
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2 protocols using anti sdha antibody
1
Immunoprecipitation and Western Blot Analysis
2
Western Blot Analysis of Protein Markers
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Medium 199 was purchased from Gibco; Thermo Fisher Scientific, Inc. Penicillin-streptomycin solution and FBS were purchased from HyClone; GE Healthcare Life Sciences. Trypsin-EDTA (0.05%) was obtained from Gibco; Thermo Fisher Scientific, Inc. Antibodies specific for β-actin (cat. no. sc-47778) and horseradish peroxidase-conjugated secondary antibodies [goat anti-mouse (cat. no. sc-2005) and anti-rabbit (cat. no. sc-2004)] were obtained from Santa Cruz Biotechnology, Inc. Anti-SDHA antibody (cat. no. ab66484) was purchased from Abcam. The primary antibody against HPRT1 (cat. no. LS-C81245) was purchased from LifeSpan BioSciences, Inc. and the anti-p53 primary antibody (cat. no. ARP37163_T100) was obtained from Aviva Systems Biology Corporation. Pifithrin-α (PFT-α; P4359) was purchased from Sigma-Aldrich; Merck KGaA (32 (link)).
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