Mead acid

Manufactured by Cayman Chemical

Sourced in United States

Mead acid is a chemical compound used in laboratory settings. It serves as a fundamental reagent for various chemical reactions and analyses. The core function of Mead acid is to facilitate specific chemical transformations and processes within the controlled environment of a laboratory.

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Lab products found in correlation

Dichloromethane, by Avantor (1 mentions) Supelco 37 component fame mix, by Merck Group (1 mentions) Arachidonic acid, by Merck Group (1 mentions) Ferrostatin 1, by Cayman Chemical (1 mentions) Bwa4c, by Merck Group (1 mentions) Erastin, by Cayman Chemical (1 mentions) α tocopherol, by Merck Group (1 mentions) Dihomo γ linolenic acid, by Cayman Chemical (1 mentions) Bay x1005, by Bio-Techne (1 mentions) Sc236, by Bio-Techne (1 mentions) Pd146176, by Bio-Techne (1 mentions) Aa 861, by Merck Group (1 mentions) Psi 7977 sofosbuvir, by ChemScene (1 mentions) Sc 560, by Bio-Techne (1 mentions) Zileuton, by Bio-Techne (1 mentions) Hcv ns3, by Abcam (1 mentions) Cay10566, by Cayman Chemical (1 mentions) Mk886, by Bio-Techne (1 mentions) Gapdh, by Fujifilm (1 mentions) Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)

2 protocols using mead acid

GC-MS Reference Standard Curve

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The gas chromatography–mass spectrometry (GC-MS) reference standard curve contained a mixture of Supelco 37 Component FAME Mix (Sigma-Aldrich, St. Louis, MO, USA) with mead acid, docosatetraenoic acid, n-3 docosapentaenoic acid (DPA), n-6 DPA, and palmitelaidic acid (Cayman Chemical, Ann Arbor, MI, USA). BCFAs were compared to Mixture BR 3 (Larodan AB, Solna, Sweden), while CLA isomers were compared to CLA reference standard UC-59M (Nu-Chek Prep, Elysian, MN, USA). Dichloromethane was purchased from VWR Chemicals (Radnor, PA, USA). All other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise noted.

Sergin S., Jambunathan V., Garg E., Rowntree J.E, & Fenton J.I. (2022). Fatty Acid and Antioxidant Profile of Eggs from Pasture-Raised Hens Fed a Corn- and Soy-Free Diet and Supplemented with Grass-Fed Beef Suet and Liver. Foods, 11(21), 3404.

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Lipoprotein Depletion and Lipid Signaling Assays

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AA-861, BWA4C, α-tocopherol, linoleic acid, arachidonic acid, dimethyl sulfoxide were from Sigma. CAY10566, Mead acid, dihomo-γ-linolenic acid, erastin, ferrostatin-1, and (1S,3R)-RSL3 were from Cayman Chemical. SC560, SC236, MK886, Zileuton, BAY-X1005, PD146176, and deferoxamine were from Tocris Bioscience. PSI-7977 (Sofosbuvir) and Glecaprevir were from Chemscene. Cell viability was determined using Cell Counting Kit-8 (DOJINDO, Japan). Lipoprotein-deprived serum (LPDS) was prepared by incubating the heat-inactivated FBS with fumed silica (Sigma, S5130) overnight, followed by removal of the silica by centrifugation at 2,000g for 20 min and filtration using a 0.22 μm filter device.
Primary antibodies to SCD (1:500 dilution, #2438) was from Cell Signaling Technology; FADS1 (1:1,000 dilution, #27533) was from Cayman Chemical; FADS2 (1:1,000 dilution, A10270) were from ABclonal; FADS2 (1:1,000 dilution, 28034–1-AP) was from Proteintech; HCV NS3 (1:500, ab13830) was from Abcam; GAPDH was from Wako (1:4,000 dilution, 016–25523). IRDye 680 or 800 secondary antibodies including #926–32211, #926–32212, #926–32214, #926–68020 and #926–68073 (1:20,000) were from LI-COR.

Yamane D., Hayashi Y., Matsumoto M., Nakanishi H., Imagawa H., Kohara M., Lemon S.M, & Ichi I. (2021). FADS2-dependent fatty acid desaturation dictates cellular sensitivity to ferroptosis and permissiveness for hepatitis C virus replication. Cell chemical biology, 29(5), 799-810.e4.

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