Acetylsalicylic acid aspirin asa

OVM, the trypsin inhibitor from chicken egg whites, and casein from cow’s milk were purchased from Nacalai Tesque (Kyoto, Japan). Acetylsalicylic acid (aspirin: ASA), lysine acetylsalicylic acid (lysine-aspirin: L-ASA) and α-casein from cow’s milk were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium dodecyl sulfate (SDS) and ethanol (EtOH) were purchased from FUJIFILM Wako Chemicals Corporation (Osaka, Japan). Mouse MCPT-1 (mMCPT-1) uncoated enzyme-linked immunosorbent assay (ELISA) kit was purchased from Invitrogen (Waltham, MA, USA).

RC-4 B/C (CRL-1903) and GH3 (CCL-82.1) cells were purchased from American Type Culture Collection (ATCC). Cells were cultured following ATCC recommendations and were used for experiments for not more than 20 passages. Acetylsalicylic acid (aspirin, ASA) was purchased from Sigma Chemical Co. (#A5376, St. Louis, MO, USA), and YM155 was obtained from Selleckchem (#S1130, Munich, Germany). Stock solutions of both compounds were prepared in dimethyl sulfoxide (DMSO). Human Recombinant TRAIL was purchased from Thermo Fisher Scientific (Waltham, MA, USA) and dissolved in nuclease-free water containing 0.1% BSA according to the manufacturer’s instructions. For cell treatment TRAIL was used in 2 ug/ml final concentration.