Ficoll histopaque density gradient centrifugation

Human primary PBMCs were isolated from whole blood of healthy donors, as previously described (Barda-Saad et al., 2005 (link)). Blood samples from anonymous healthy donors were provided by Magen David Adom (MDA; Israeli National Blood Bank). Informed consent was obtained from all donors. The experiments conformed to the principles set out in the WMA Declaration of Helsinki and the Department of Health and Human Services Belmont Report. The research was performed with approval of and according to the guidelines of the Bar-Ilan University Ethics Committee. Briefly, human PBMCs were isolated from whole blood by Ficoll-Histopaque density gradient centrifugation (MP Biomedical).

Human primary PBMCs were isolated from whole blood of healthy donors, as previously described (Barda‐Saad et al, 2005 (link)). Blood samples from healthy donors were randomly collected and provided by Magen David Adom (MDA; Israeli National Blood Bank). Informed consent was obtained from all donors. Donor's identification information remained anonymous. The experiments conformed to the principles set out in the WMA Declaration of Helsinki and the Department of Health and Human Services Belmont Report. The research was performed with approval of and according to the guidelines of the Bar‐Ilan University Ethics committee. Briefly, human PBMCs were isolated from whole blood by Ficoll‐Histopaque density gradient centrifugation (MP Biomedical). Isolated cells were cultured in RPMI 1640 (Sigma‐Aldrich) supplemented with 10% FBS (Biological industries). All cells were grown at 37°C, under an atmosphere containing 5% CO₂.