Carvacrol oil (5-isopropryl-2-methylphenol, purity 98%), sorbitan monostearate 80 (Span 80®), and Tiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma–Aldrich (St. Louis, MO, United States). Polysorbate 80 (Tween 80®) was provided by Vetec Química Fina LTDA (Duque de Caxias, RJ, Brazil). Medium chain triglyceride (Mygliol 812®) was acquired from Sasol (Brunsbüttel, Germany). RPMI 1,640 culture medium Dulbecco’s Modifed Eagle Medium (DMEM), inactivated fetal bovine serum and antibiotics (10,000 units/ml Penicillin G sodium salt and 10 mg/ml streptomycin sulfate) were purchased from Vitrocell (Campinas, SP, Brazil). Praziquantel was kindly provided by Ecovet Indústria Veterinária Ltda (São Paulo, Brazil).
Penicillin g sodium salt
Manufactured by Vitrocell
Sourced in Brazil
Penicillin G sodium salt is a white crystalline powder that is a common antibiotic compound. It functions as a bactericidal agent by inhibiting the synthesis of bacterial cell walls.
Automatically generated - may contain errors
Lab products found in correlation
Hepes buffer, by Merck Group (2 mentions)
Penicillin g streptomycin, by Vitrocell (2 mentions)
Thiazolyl blue tetrazolium bromide mtt, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Mygliol 812, by Sasol (1 mentions)
Tiazolyl blue tetrazolium bromide, by Merck Group (1 mentions)
Span 80, by Merck Group (1 mentions)
Streptomycin sulfate, by Vitrocell (1 mentions)
Dulbecco s modified eagle medium dmem, by Vitrocell (1 mentions)
Rpmi 1640 medium, by Vitrocell (1 mentions)
3 protocols using penicillin g sodium salt
1
Carvacrol Oil Cytotoxicity Evaluation
2
Antiparasitic Compound Screening Protocol
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RPMI 1640 medium, Dulbecco’s modified Eagle medium (DMEM), heat-inactivated fetal calf serum, and penicillin G-streptomycin solutions (10,000 U/mL penicillin G sodium salt and 10 mg/mL streptomycin sulfate) were obtained from Vitrocell (Campinas, SP, Brazil). HEPES buffer, dimethyl sulfoxide (DMSO), and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma (St. Louis, MO). Praziquantel was kindly provided by Ecovet Indústria Veterinária Ltda (São Paulo, SP, Brazil). NFZ was synthesized as describe in the published protocols (22 (link), 51 (link)). In all in vitro experiments, compounds were solubilized in DMSO.
3
In Vitro Cytotoxicity Evaluation of Praziquantel
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Dulbecco’s Modified Eagle Medium (DMEM) modified to contain 4 mM of L-glutamine, 4500 mg/L glucose, and 1 mM sodium pyruvate, Roswell Park Memorial Institute (RPMI) 1640 medium, trypsin/EDTA solutions, heat-inactivated fetal calf serum, and penicillin G-streptomycin solutions (10,000 U/ml penicillin G sodium salt, 10 mg/ml streptomycin sulfate) were obtained from Vitrocell (Campinas, SP, Brazil). HEPES buffer, thiazolyl blue tetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO) were purchased from Sigma (St. Louis, MO, USA). Praziquantel was kindly provided by Ecovet Indústria Veterinária Ltda (São Paulo, SP, Brazil). In all in vitro experiments, compounds were solubilized in DMSO.
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