Goat antihuman fcγ

Manufactured by Jackson ImmunoResearch

Goat–antihuman Fcγ is a laboratory reagent used to detect and quantify human immunoglobulins. It is a polyclonal antibody produced in goats that specifically binds to the Fc region of human IgG antibodies. This reagent can be used in various immunoassays and other applications that require the detection or measurement of human IgG.

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Lab products found in correlation

Doxycycline hydrochloride, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Harvard Bioscience (1 mentions) Fetal bovine serum (fbs), by Harvard Bioscience (1 mentions) Glutamine, by Harvard Bioscience (1 mentions) Hygromycin b, by Carl Roth (1 mentions) Blasticidin s, by InvivoGen (1 mentions) Atp disodium salt hydrate, by Merck Group (1 mentions) Adp sodium salt, by Merck Group (1 mentions) Medetomidine, by Pfizer (1 mentions) Dormitor, by Pfizer (1 mentions) Lsrfortessa x 20 flow cytometer, by BD (1 mentions)

2 protocols using goat antihuman fcγ

Recombinant Protein Purification and Characterization

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Standard laboratory chemicals were purchased from Carl Roth. Ham's F‐12 growth medium, fetal bovine serum, PBS, and glutamine were from Biochrom. Blasticidin S was obtained from InvivoGen. Hygromycin B came from Carl Roth. ProCHO4 growth medium was from Lonza. All enzymes for cloning were bought from New England Biolabs. Doxycycline hydrochloride, ADP sodium salt, and ATP disodium salt hydrate were purchased from Sigma‐Aldrich. Part of the recombinant hirudin was a kind gift from Prof. Christian P. Sommerhoff (University of Munich), and another part was purchased from Celgene. Collagen‐related peptide was synthesized at AnaSpec and chemically cross‐linked. DiOC6 (3,3′‐dihexyloxacarbocyanine iodide) was from Life Technologies. midazolam (Dormicum; Roche) was purchased from Roche, and medetomidine (Dormitor; Pfizer) and fentanyl were both from Janssen‐Cilag. Recombinant soluble human CD39 (solCD39) was obtained from R&D Systems. Goat–antihuman Fcγ and goat–antihuman IgG (H+L)‐POD (peroxidase) were purchased from Jackson ImmunoResearch.

Degen H., Borst O., Ziegler M., Mojica Munoz A., Jamasbi J., Walker B., Göbel S., Fassbender J., Adler K., Brandl R., Münch G., Lorenz R., Siess W., Gawaz M, & Ungerer M. (2017). ADPase CD39 Fused to Glycoprotein VI‐Fc Boosts Local Antithrombotic Effects at Vascular Lesions. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 6(8), e005991.

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Anti-CD20 Antibody Binding Assay

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Anti-hCD20 Abs diluted in DPBS/1% bovine serum albumin (BSA) were incubated with 6×10⁵ Raji cells in V-bottom 96-well plates at 4°C for 1 hr in a final volume of 50 μL. The cells were washed with cold DPBS/1% BSA and incubated at 4°C for 1 hr with 50 μg/mL goat anti-human Fcγ (Jackson ImmunoResearch) labeled with Atto 488 (A488) NHS ester as previously described [27 (link)]. Binding of mAbs to live single cells gated by light scatter characteristics was measured using a BD LSRFortessa X-20 flow cytometer. Representative flow cytometry histograms are presented in Supplementary Figure S1. The binding of bacteriophage displaying the Fab portions of the chicken Abs to hCD20-transfected Chinese hamster ovary (CHO) cells was also measured (Supplementary Figure S2).

Chockalingam K., Kumar A., Song J, & Chen Z. (2022). Chicken-derived CD20 antibodies with potent B cell depletion activity. British journal of haematology, 199(4), 560-571.

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