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Z gel serum separation microtubes

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The Z‐Gel serum separation microtubes are a type of laboratory equipment used to separate serum from whole blood samples. These tubes contain a gel barrier that allows for the effective separation of serum from the cellular components of the blood sample during centrifugation.

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Z-Gel microtubes Serum Z Z-Gel Serum gel Microtubes

2 protocols using z gel serum separation microtubes

1

Cytokine Profiling of Transplant Recipients

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Peripheral blood was collected on D7 from BMT recipients in 1.1‐mL Z‐Gel serum separation microtubes (Sarstedt, Newton, North Carolina). Following serum separation, enzyme‐linked immunosorbent assay (ELISA) was performed for IFNγ, TNFα, and Prostaglandin E2 (PGE2). Mixed leukocyte culture (MLC) supernatants were harvested at 48 or 72 hours as indicated and then assayed for mouse cytokines (interleukin [IL]‐10, Transforming Growth Factor Beta (TGFβ), IFNγ, TNFα, and PGE2) according to the manufacturer instructions (R&D Systems, Inc. Minneapolis, MN and eBioScience).
Metheny L., Eid S., Wuttisarnwattana P., Auletta J.J., Liu C., Van Dervort A., Paez C., Lee Z., Wilson D., Lazarus H.M., Deans R., Vant Hof W., Ktena Y, & Cooke K.R. (2021). Human multipotent adult progenitor cells effectively reduce graft‐vs‐host disease while preserving graft‐vs‐leukemia activity. Stem Cells (Dayton, Ohio), 39(11), 1506-1519.
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2

Quantifying Cytokine Responses in BMT

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Peripheral blood was collected on D7 from BMT recipients in 1.1-mL Z-Gel serum separation microtubes (Sarstedt, Newton, North Carolina). Following serum separation, enzyme-linked immunosorbent assay (ELISA) was performed for IFNγ, TNFα, and Prostaglandin E2 (PGE2). Mixed leukocyte culture (MLC) supernatants were harvested at 48 or 72 hours as indicated and then assayed for mouse cytokines (interleukin [IL]-10, Transforming Growth Factor Beta (TGFβ), IFNγ, TNFα, and PGE2) according to the manufacturer instructions (R&D Systems, Inc. Minneapolis, MN and eBioScience).
Metheny L., Eid S., Wuttisarnwattana P., Auletta J.J., Liu C., Van Dervort A., Paez C., Lee Z., Wilson D., Lazarus H.M., Deans R., Vant Hof W., Ktena Y, & Cooke K.R. (2021). Human multipotent adult progenitor cells effectively reduce graft‐vs‐host disease while preserving graft‐vs‐leukemia activity. Stem Cells (Dayton, Ohio), 39(11), 1506-1519.
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