Flow cytometry was carried out by suspending MNCs in the stain buffer and treated first with FcR blocking reagent (1:100, Miltenyi Biotech) followed by incubation with the following fluorescent-conjugated antibodies (Biolegend), allophycocyanin (APC) anti-human lineage cocktail (1:500, Biolegend), phycoerythrin (PE) anti-human CD45 (1:500) and Fluorescein isothiocyanate (FITC) anti-human CD34 antibodies (1 in 250) or isotype control antibodies (1 in 500) (Biolegend, San Diego, CA, U.S.A.) for 45 min at 4°C. Dead cells were excluded using 7-AAD viability staining solution. Flow cytometry was carried out by using C6 Accuri cytometer (BD).
Allophycocyanin apc anti human lineage cocktail
Manufactured by BioLegend
Sourced in United States
Allophycocyanin (APC) anti-human lineage cocktail is a fluorescent-labeled antibody reagent that binds to a combination of lineage-specific surface markers expressed on human cells. It is designed for use in flow cytometry applications to identify and characterize different cell types.
Automatically generated - may contain errors
Lab products found in correlation
Fcr blocking reagent, by Miltenyi Biotec (2 mentions)
Fluorescein isothiocyanate fitc anti human cd34 antibodies, by BioLegend (2 mentions)
Accuri c6 cytometer, by BD (2 mentions)
Fluorescent conjugated antibodies, by BioLegend (2 mentions)
Anti human cd45, by BioLegend (1 mentions)
Isotype control antibody, by BioLegend (1 mentions)
2 protocols using allophycocyanin apc anti human lineage cocktail
1
Quantifying Hematopoietic Stem Cells
2
Flow Cytometry Characterization of MNCs
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Flow cytometry was carried out by suspending MNCs in the stain buffer and treated first with FcR blocking reagent (1:100, Miltenyi Biotec) followed by incubation with the following fluorescent-conjugated antibodies (Biolegend), allophycocyanin (APC) anti-human lineage cocktail (1:500, Biolegend), phycoerythrin (PE) anti-human CD45 (1:500) and fluorescein isothiocyanate (FITC) anti-human CD34 antibodies (1 in 250), or isotype control antibodies (1 in 500) (Biolegend, SanDiego, CA, USA) for 45 min at 4 °C. Dead cells were excluded using the 7-AAD viability staining solution. Flow cytometry was carried out by using the C6 Accuri cytometer (BD).
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