STAT3 reporter HeLa stable cell lines (Signosis Inc, Santa Clara, CA) were incubated in a 96-well microplate for 24 h. Cells were pretreated with test compounds for 2 h, and 10 ng/ml (w/v) of oncostatin M were applied and incubated for 4 h. Cells were washed with medium not supplemented with phenol red, and Steady-Glo® reagent (Promega, Madison, WI) was applied. After 15 min incubation, the signals were detected by ARVO Light 1420 (PerkinElmer Life Sciences, Waltham, MA). The relative signal intensity was calculated in each well as the ratio for the mean signal of vehicle.
Arvo light 1420
Manufactured by PerkinElmer
The ARVO Light 1420 is a multimode microplate reader that can measure absorbance, fluorescence, and luminescence. It is designed to support a wide range of applications in life science research and drug discovery.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using arvo light 1420
1
STAT3 Signaling Pathway Activation Assay
2
Cytokine-Mediated NK Cell Activation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A172 cells were seeded in 96-well plates and incubated for 24 hours. Medium was
changed to 500 μM L-Trp-containing culture medium of NK-92MI and test compounds
were added to the wells. After treating for 1 hour with the test compound, IFN-γ
was added to each well. The final concentration of IFN-γ was 10 ng/mL. Cells
were cultured for 48 hours and then the supernatant of the culture medium was
collected as conditioned medium. NK-92MI cells were seeded in 96-well plates and
cultured in the conditioned medium. After 72 hours, NK-92MI cells were used for
cell proliferation, cell killing activity. In the cell proliferation assay,
CellTiter-Glo (Promega, Madison, WI) was added to each well and the luminescence
was measured by ARVO Light 1420 (PerkinElmer, Waltham, MA). In the cell killing
activity assay, HeLa cells were pre-cultured for 24 hours and then cultured with
NK-92MI cells for 5 hours, then NK-92MI cells were washed out and proliferation
of the HeLa cells was measured using the Cell viability assay. In the Granzyme B
production assay, NK-92MI cells were pre-cultured for 72 hours with Kyn and then
cultured with fresh medium without Kyn for 5.5 hours, then the culture
supernatant was collected. The granzyme B concentration in the supernatants was
evaluated using a human Granzyme B ELISA kit (Diaclone, Besancon, France),
according to a protocol recommended by the manufacturer.
changed to 500 μM L-Trp-containing culture medium of NK-92MI and test compounds
were added to the wells. After treating for 1 hour with the test compound, IFN-γ
was added to each well. The final concentration of IFN-γ was 10 ng/mL. Cells
were cultured for 48 hours and then the supernatant of the culture medium was
collected as conditioned medium. NK-92MI cells were seeded in 96-well plates and
cultured in the conditioned medium. After 72 hours, NK-92MI cells were used for
cell proliferation, cell killing activity. In the cell proliferation assay,
CellTiter-Glo (Promega, Madison, WI) was added to each well and the luminescence
was measured by ARVO Light 1420 (PerkinElmer, Waltham, MA). In the cell killing
activity assay, HeLa cells were pre-cultured for 24 hours and then cultured with
NK-92MI cells for 5 hours, then NK-92MI cells were washed out and proliferation
of the HeLa cells was measured using the Cell viability assay. In the Granzyme B
production assay, NK-92MI cells were pre-cultured for 72 hours with Kyn and then
cultured with fresh medium without Kyn for 5.5 hours, then the culture
supernatant was collected. The granzyme B concentration in the supernatants was
evaluated using a human Granzyme B ELISA kit (Diaclone, Besancon, France),
according to a protocol recommended by the manufacturer.
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