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Rt 2 mouse cytokines and chemokines array pamm 150z

Manufactured by Qiagen
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The Qiagen RT (2) Mouse Cytokines and Chemokines array (PAMM-150Z) is a real-time PCR array designed to analyze the expression of 84 genes related to mouse cytokines and chemokines.

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Lab products found in correlation

Rt2 first strand kit, by Qiagen (2 mentions) Quantstudio 5 real time pcr instrument, by Thermo Fisher Scientific (2 mentions) Nanovue plus, by GE Healthcare (1 mentions)

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PAMM-150Z (3 citations)

2 protocols using rt 2 mouse cytokines and chemokines array pamm 150z

1

Quantifying Mouse Cytokine Expression

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The concentration of lung RNA was quantified using a Nanodrop (NanoVue Plus, GE Healthcare), and 400 ng of RNA was converted to cDNA using the Qiagen RT (2 ) First Strand Kit (330404; Qiagen). The cDNA was analyzed with the Qiagen RT (2 ) Mouse Cytokines and Chemokines array (PAMM-150Z; Qiagen). Reactions were run on a QuantStudio 5 Real-Time PCR Instrument (A28133; Applied Biosystems). The results were analyzed with the Qiagen analysis spreadsheet provided with the kit.
McGrath M.E., Xue Y., Dillen C., Oldfield L., Assad-Garcia N., Zaveri J., Singh N., Baracco L., Taylor L.J., Vashee S, & Frieman M.B. (2022). SARS-CoV-2 variant spike and accessory gene mutations alter pathogenesis. Proceedings of the National Academy of Sciences of the United States of America, 119(37), e2204717119.
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2

Lung RNA Cytokine Analysis

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The concentration of lung RNA was quantified using a Nanodrop (NanoVue Plus). 400ng of RNA was converted to cDNA using the Qiagen RT2 First Strand Kit (330404, Qiagen, Hilden, Germany). The cDNA was analyzed with the Qiagen RT2 Mouse Cytokines and Chemokines array (PAMM-150Z, Qiagen, Hilden, Germany). Reactions were run on a QuantStudio 5 Real-Time PCR Instrument (A28133, Applied Biosystems, Waltham, MA). The results were analyzed with the Qiagen analysis spreadsheet provided with the kit.
McGrath M.E., Xue Y., Dillen C., Oldfield L., Assad-Garcia N., Zaveri J., Singh N., Baracco L., Taylor L., Vashee S, & Frieman M. (2022). SARS-CoV-2 Variant Spike and accessory gene mutations alter pathogenesis. bioRxiv.
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