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Sigma variable pressure field emission scanning electron microscope

Manufactured by Zeiss
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Sourced in United Kingdom

The Sigma variable pressure field emission scanning electron microscope is a high-performance imaging and analysis tool designed for a wide range of applications. It utilizes a field emission electron source and variable pressure capabilities to provide high-resolution images and data on the surface characteristics of various materials and samples.

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Lab products found in correlation

Amira software, by Thermo Fisher Scientific (2 mentions) Gatan 3view serial block face imaging system, by Ametek (2 mentions)

Close spelling variants of this product

Sigma Variable Pressure Scanning Electron Microscope Sigma field emission scanning electron microscope Field emission scanning electron microscope Sigma scanning electron microscope Scanning electron microscope Sigma microscope

2 protocols using sigma variable pressure field emission scanning electron microscope

1

Serial Block Face Imaging of Retinal Tissue

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Tissue was processed for serial block face scanning electron microscopy (SEM) using an adapted version of a protocol established by Deerinck et al 20105 (link). Whole eyes were isolated and fixed in Karnovsky's fixative. The cornea and lens were removed and the tissue further fixed in tannic acid overnight. Heavy metal infiltration was then undertaken; tissue was incubated in 1.5% potassium ferrocyanide, 0.5% osmium tetroxide in cacodylate buffer, followed by thiocarbohydrazide treatment and a second exposure to 1% osmium incubation. Walton’s lead aspartate exposure was not carried out, so they were finished with 1% uranyl acetate incubation followed by dehydration to propylene oxide and embedded in Durcupan ACM resin. The tissue was serially sectioned and imaged using the Gatan 3VIEW serial block face imaging system (Gatan, Abingdon, UK) fitted to a Zeiss Sigma variable pressure field emission scanning electron microscope (Zeiss, Cambridge, UK). Data was collected and used in Amira Software (FEI, Oregon, USA) in order to reconstruct the 3D images. Using the same software, photoreceptor mitochondrial volume was estimated for WT mice, around the lesions in Vldlr−/− mice, and away from the lesion in Vldlr−/− mice.
Joyal J.S., Sun Y., Gantner M.L., Shao Z., Evans L.P., Saba N., Fredrick T., Burnim S., Kim J.S., Patel G., Juan A.M., Hurst C.G., Hatton C.J., Cui Z., Pierce K.A., Bherer P., Aguilar E., Powner M.B., Vevis K., Boisvert M., Fu Z., Levy E., Fruttiger M., Packard A., Rezende F.A., Maranda B., Sapieha P., Chen J., Friedlander M., Clish C.B, & Smith L.E. (2016). Retinal lipid and glucose metabolism dictates angiogenesis through lipid sensor Ffar1. Nature medicine, 22(4), 439-445.
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2

Serial Block Face Imaging of Retinal Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue was processed for serial block face scanning electron microscopy (SEM) using an adapted version of a protocol established by Deerinck et al 20105 (link). Whole eyes were isolated and fixed in Karnovsky's fixative. The cornea and lens were removed and the tissue further fixed in tannic acid overnight. Heavy metal infiltration was then undertaken; tissue was incubated in 1.5% potassium ferrocyanide, 0.5% osmium tetroxide in cacodylate buffer, followed by thiocarbohydrazide treatment and a second exposure to 1% osmium incubation. Walton’s lead aspartate exposure was not carried out, so they were finished with 1% uranyl acetate incubation followed by dehydration to propylene oxide and embedded in Durcupan ACM resin. The tissue was serially sectioned and imaged using the Gatan 3VIEW serial block face imaging system (Gatan, Abingdon, UK) fitted to a Zeiss Sigma variable pressure field emission scanning electron microscope (Zeiss, Cambridge, UK). Data was collected and used in Amira Software (FEI, Oregon, USA) in order to reconstruct the 3D images. Using the same software, photoreceptor mitochondrial volume was estimated for WT mice, around the lesions in Vldlr−/− mice, and away from the lesion in Vldlr−/− mice.
Joyal J.S., Sun Y., Gantner M.L., Shao Z., Evans L.P., Saba N., Fredrick T., Burnim S., Kim J.S., Patel G., Juan A.M., Hurst C.G., Hatton C.J., Cui Z., Pierce K.A., Bherer P., Aguilar E., Powner M.B., Vevis K., Boisvert M., Fu Z., Levy E., Fruttiger M., Packard A., Rezende F.A., Maranda B., Sapieha P., Chen J., Friedlander M., Clish C.B, & Smith L.E. (2016). Retinal lipid and glucose metabolism dictates angiogenesis through lipid sensor Ffar1. Nature medicine, 22(4), 439-445.
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