Pyrrole

Benzaldehyde-2-sulfonic acid sodium salt (BS), chloranil, 2-methylresorcinol (2,6-dihydroxytoluene) (DHT), and 2,4,6-trihydroxybenzaldehyde (THBA) were purchased from Tokyo Chemical Industry (Tokyo, Japan). Pyrrole (Pyr), pyrogallol (1,2,3-trihydroxybenzene, THB), acetyl chloride, triethylamine, p-toluenesulfonic acid monohydrate (p-TS), tetrabutylammonium hexafluorophosphate, acetonitrile, and phosphate buffer were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). The Pyr monomer was purified via distillation prior to use.

All chemical reagents were of analytical grade, used as supplied without further purification unless indicated. Pyrrole, KH₂PO₄, H₃PO4, lysozyme, sodium dodecyl sulfate (SDS), and NaOH were purchased from Wako Pure Chemical Industries (Japan). Nutrient broth was purchased from Eiken Chemical (Japan). Bacterial viability kit L7007 (SYTO9/PI) was purchased from Molecular Probes. Bacteria such as E. coli O157:H7 and E. coli O26:H11 were provided by Prof. Miyake in Osaka Prefecture University. On the other hand, Salmonella enterica, Vibrio parahaemolyticus, Staphylococcus aureus, and Bacillus subtilis were purchased from the Biological Resource Center (NBRC), National Institute of Technology and Evaluation, Japan. Milli-Q grade (>18 MΩ) water with ultraviolet sterilization was used throughout the study.