Pgipz ns

Manufactured by Thermo Fisher Scientific

The PGIPZ-Ns is a piece of laboratory equipment designed for general laboratory use. It serves a core function of performing a specific task, but further details on its intended use or capabilities are not available.

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Lab products found in correlation

Gipz human huwe1 shrna, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Lentiviral shRNA pGIPZ vectors; NS (RHS4348) PGIPZ

2 protocols using pgipz ns

Lentiviral Transduction of mule-targeting shRNA

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Cells from male control were lentivirally transduced with plasmids carrying the non-specific control shRNA (pGIPZ-Ns, Open Biosystems) or the mule-targeting shRNA (GIPZ Human HUWE1 shRNA, Clone-ID V3LHS_353153, Open Biosystems). Stable clones were subsequently selected with puromycin, while the selection efficiency was monitored by the percentage of green fluorescent protein (GFP)-positive cells.

Friez M.J., Brooks S.S., Stevenson R.E., Field M., Basehore M.J., Adès L.C., Sebold C., McGee S., Saxon S., Skinner C., Craig M.E., Murray L., Simensen R.J., Yap Y.Y., Shaw M.A., Gardner A., Corbett M., Kumar R., Bosshard M., van Loon B., Tarpey P.S., Abidi F., Gecz J, & Schwartz C.E. (2016). HUWE1 mutations in Juberg-Marsidi and Brooks syndromes: the results of an X-chromosome exome sequencing study. BMJ Open, 6(4), e009537.

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Genetic Manipulation of Mule in LCLs

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Wild-type (WT) LCLs were lentivirally transduced with plasmids carrying the non-specific control shRNA (pGIPZ-Ns, Open Biosystems) or the Mule-targeting shRNA (GIPZ Human HUWE1 shRNA, Clone-ID V3LHS_353153, TCCTCTGTACCAACAACCT, Open Biosystems). Stable clones were subsequently selected with puromycin (1 µg/ml), while the selection-efficiency was monitored by the percentage of GFP-positive cells.

Bosshard M., Aprigliano R., Gattiker C., Palibrk V., Markkanen E., Backe P.H., Pellegrino S., Raymond F.L., Froyen G., Altmeyer M., Bjørås M., Dianov G.L, & van Loon B. (2017). Impaired oxidative stress response characterizes HUWE1-promoted X-linked intellectual disability. Scientific Reports, 7, 15050.

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